@article{PAR00013494,
  title = {{R}apid diagnosis of tuberculosis by real-time high-resolution imaging of {M}ycobacterium tuberculosis colonies},
  author = {{G}hodbane, {R}. and {A}smar, {S}. and {B}etzner, {M}. and {L}inet, {M}. and {P}ierquin, {J}. and {R}aoult, {D}idier and {D}rancourt, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}ulture remains the cornerstone of diagnosis for pulmonary tuberculosis, but the fastidiousness of {M}ycobacterium tuberculosis may delay culture-based diagnosis for weeks. {W}e evaluated the performance of real-time high-resolution imaging for the rapid detection of {M}. tuberculosis colonies growing on a solid medium. {A} total of 50 clinical specimens, including 42 sputum specimens, 4 stool specimens, 2 bronchoalveolar lavage fluid specimens, and 2 bronchial aspirate fluid specimens were prospectively inoculated into (i) a commercially available {M}iddlebrook broth and evaluated for mycobacterial growth indirectly detected by measuring oxygen consumption (standard protocol) and (ii) a home-made solid medium incubated in an incubator featuring real-time high-resolution imaging of colonies (real-time protocol). {I}solates were identified by {Z}iehl-{N}eelsen staining and matrix-assisted laser desorption ionization-time of flight mass spectrometry. {U}se of the standard protocol yielded 14/50 (28%) {M}. tuberculosis isolates, which is not significantly different from the 13/50 (26%) {M}. tuberculosis isolates found using the real-time protocol ({P} = 1.00 by {F}isher's exact test), and the contamination rate of 1/50 (2%) was not significantly different from the contamination rate of 2/50 (4%) using the real-time protocol ({P} = 1.00). {T}he real-time imaging protocol showed a 4.4-fold reduction in time to detection, 82 +/- 54 h versus 360 +/- 142 h ({P}<0.05). {T}hese preliminary data give the proof of concept that real-time high-resolution imaging of {M}. tuberculosis colonies is a new technology that shortens the time to growth detection and the laboratory diagnosis of pulmonary tuberculosis.},
  keywords = {},
  booktitle = {},
  journal = {{J}ournal of {C}linical {M}icrobiology},
  volume = {53},
  numero = {8},
  pages = {2693--2696},
  ISSN = {0095-1137},
  year = {2015},
  DOI = {10.1128/jcm.00684-15},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00013494},
}