Publications des scientifiques de l'IRD

Salez N., Vabret A., Leruez-Ville M., Andreoletti L., Carrat F., Renois F., de Lamballerie Xavier. (2015). Evaluation of four commercial multiplex molecular tests for the diagnosis of Acute Respiratory Infections. Plos One, 10 (6), p. e0130378. ISSN 1932-6203.

Titre du document
Evaluation of four commercial multiplex molecular tests for the diagnosis of Acute Respiratory Infections
Année de publication
2015
Type de document
Article référencé dans le Web of Science WOS:000356932500096
Auteurs
Salez N., Vabret A., Leruez-Ville M., Andreoletti L., Carrat F., Renois F., de Lamballerie Xavier
Source
Plos One, 2015, 10 (6), p. e0130378 ISSN 1932-6203
Acute Respiratory Infections (ARIs) are responsible for considerable morbidity and mortality worldwide. Documentation of respiratory specimens can help for an appropriate clinical management with a significant effect on the disease progress in patient, the antimicrobial therapy used and the risk of secondary spread of infection. Here, we compared the performances of four commercial multiplex kits used in French University Hospital diagnostic microbiology laboratories for the detection of ARI pathogens (i.e., the xTAG Respiratory Viral Panel Fast, RespiFinder SMART 22, CLART PneumoVir and Fast Track Diagnostics Respiratory Pathogen 33 kits). We used a standardised nucleic acids extraction protocol and a comprehensive comparative approach that mixed reference to well established real-time PCR detection techniques and analysis of convergent positive results. We tested 166 respiratory clinical samples and identified a global high degree of correlation for at least three of the techniques (xTAG, RespiFinder and FTD33). For these techniques, the highest Youden's index (YI), positive predictive (PPV) and specificity (Sp) values were observed for Core tests (e.g., influenza A [YI:0.86-1.00; PPV:78.95-100.00; Sp:97.32-100.00] & B [YI:0.44-1.00; PPV:100.00; Sp:100.00], hRSV [YI:0.50-0.99; PPV:85.71-100.00; Sp:99.38-100.00], hMPV [YI:0.71-1.00; PPV:83.33-100.00; Sp:99.37-100.00], EV/hRV [YI:0.62-0.82; PPV:93.33-100.00; Sp:94.48-100.00], AdV [YI:1.00; PPV:100.00; Sp:100.00] and hBoV [YI:0.20-0.80; PPV:57.14-100.00; Sp:98.14-100.00]). The present study completed an overview of the multiplex techniques available for the diagnosis of acute respiratory infections.
Plan de classement
Santé : généralités [050]
Description Géographique
FRANCE
Localisation
Fonds IRD
Identifiant IRD
PAR00013489
Contact