@article{PAR00013361,
  title = {{F}austovirus, an asfarvirus-related new lineage of giant viruses infecting amoebae},
  author = {{R}eteno, {D}. {G}. and {B}enamar, {S}. and {K}halil, {J}. {B}. and {A}ndreani, {J}. and {A}rmstrong, {N}. and {K}lose, {T}. and {R}ossmann, {M}. and {C}olson, {P}. and {R}aoult, {D}idier and {L}a {S}cola, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{G}iant viruses are protist-associated viruses belonging to the proposed order {M}egavirales; almost all have been isolated from {A}canthamoeba spp. {T}heir isolation in humans suggests that they are part of the human virome. {U}sing a high-throughput strategy to isolate new giant viruses from their original protozoan hosts, we obtained eight isolates of a new giant viral lineage from {V}ermamoeba vermiformis, the most common free-living protist found in human environments. {T}his new lineage was proposed to be the faustovirus lineage. {T}he prototype member, faustovirus {E}12, forms icosahedral virions of approximate to 200 nm that are devoid of fibrils and that encapsidate a 466-kbp genome encoding 451 predicted proteins. {O}f these, 164 are found in the virion. {P}hylogenetic analysis of the core viral genes showed that faustovirus is distantly related to the mammalian pathogen {A}frican swine fever virus, but it encodes approximate to 3 times more mosaic gene complements. {A}bout two-thirds of these genes do not show significant similarity to genes encoding any known proteins. {T}hese findings show that expanding the panel of protists to discover new giant viruses is a fruitful strategy. {IMPORTANCE} {B}y using {V}ermamoeba, a protist living in humans and their environment, we isolated eight strains of a new giant virus that we named faustovirus. {T}he genomes of these strains were sequenced, and their sequences showed that faustoviruses are related to but different from the vertebrate pathogen {A}frican swine fever virus ({ASFV}), which belongs to the family {A}sfarviridae. {M}oreover, the faustovirus gene repertoire is approximate to 3 times larger than that of {ASFV} and comprises approximately two-thirds {ORF}ans (open reading frames [{ORF}s] with no detectable homology to other {ORF}s in a database).},
  keywords = {},
  booktitle = {},
  journal = {{J}ournal of {V}irology},
  volume = {89},
  numero = {13},
  pages = {6585--6594},
  ISSN = {0022-538{X}},
  year = {2015},
  DOI = {10.1128/jvi.00115-15},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00013361},
}