@article{PAR00013359,
  title = {{W}elcome to pandoraviruses at the '{F}ourth {TRUC}' club},
  author = {{S}harma, {V}. and {C}olson, {P}. and {C}habrol, {O}. and {S}cheid, {P}. and {P}ontarotti, {P}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{N}ucleocytoplasmic large {DNA} viruses, or representatives of the proposed order {M}egavirales, belong to families of giant viruses that infect a broad range of eukaryotic hosts. {M}egaviruses have been previously described to comprise a fourth monophylogenetic {TRUC} (things resisting uncompleted classification) together with cellular domains in the universal tree of life. {R}ecently described pandoraviruses have large (1.9-2.5 {MB}) and highly divergent genomes. {I}n the present study, we updated the classification of pandoraviruses and other reported giant viruses. {P}hylogenetic trees were constructed based on six informational genes. {H}ierarchical clustering was performed based on a set of informational genes from {M}egavirales members and cellular organisms. {H}omologous sequences were selected from cellular organisms using {T}ime{T}ree software, comprising comprehensive, and representative sets of members from {B}acteria, {A}rchaea, and {E}ukarya. {P}hylogenetic analyses based on three conserved core genes clustered pandoraviruses with phycodnaviruses, exhibiting their close relatedness. {A}dditionally, hierarchical clustering analyses based on informational genes grouped pandoraviruses with {M}egavirales members as a super group distinct from cellular organisms. {T}hus, the analyses based on core conserved genes revealed that pandoraviruses are new genuine members of the '{F}ourth {TRUC}' club, encompassing distinct life forms compared with cellular organisms.},
  keywords = {giant virus ; {P}andoravirus ; nucleocytoplasmic large {DNA} viruses ; {M}egavirales ; informational genes ; domains of life ; {TRUC} ; phylogeny},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {6},
  numero = {},
  pages = {art. 423 [11 p.]},
  ISSN = {1664-302{X}},
  year = {2015},
  DOI = {10.3389/fmicb.2015.00423},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00013359},
}