@article{PAR00013206,
  title = {{N}ew {B}orrelia species detected in ixodid ticks in {O}romia, {E}thiopia},
  author = {{K}umsa, {B}. and {S}ocolovschi, {C}. and {R}aoult, {D}idier and {P}arola, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{L}ittle is known about {B}orrelia species transmitted by hard ticks in {E}thiopia. {T}he present study was conducted from {N}ovember 2011 through {M}arch 2014 to address the occurrence and molecular identity of these bacteria in ixodid ticks infesting domestic animals in {O}romia, {E}thiopia. {A} total of 767 ixodid ticks collected from domestic animals were screened for {B}orrelia {DNA} by quantitative (q) real-time {PCR} followed by standard {PCR} and sequencing to identify the species. {O}verall, 3.8% (29/767) of the tested ticks were positive for {B}orrelia {DNA}, including 8/119 (6.7%) {A}mblyomma cohaerens, 1/42 (2.4%) {A}m. gemma, 3/53 (5.7%){A}m. variegatum, 5/22 (22.7%){A}mblyomma larvae, 3/60 (5%){A}mblyomma nymphs, 2/139(1.4%) {R}hipicephalus ({B}oophilus) decoloratus, 2/31 (6.4%) {R}h. decoloratus nymphs, and 5/118 (4.2%) {R}h. pulchellus using 16{S} genus-specific q{PCR}. {T}he prevalence of {B}orrelia {DNA} was significantly higher in genus {A}mblyomma (20/298, 6.7%) than in the genus {R}hipicephalus (9/417, 2.1%) ticks ({P} = 0.001). {S}equencing of {PCR} products from the fla{B} and 16{S} r{RNA} genes of {B}orrelia spp. from {A}mblyomma ticks showed the presence of a new species between the relapsing fever and {L}yme disease groups. {H}owever, {B}orrelia sp. detected in {R}hipicephalus ticks clustered with {B}. theileri/{B}. lonestari. {T}he human pathogenicity of the {B}orrelia sp. detected in {A}mblyomma ticks from {E}thiopia has not yet been investigated, whereas the {B}orrelia sp. detected in {R}hipicephalus ticks in our study is the causative agent of bovine borreliosis in cattle and may have veterinary importance in different parts of {E}thiopia. {F}urthermore, the detection of previously unrecognized {B}orrelia species in {A}mblyomma and {R}hipicephalus ticks in {E}thiopia generates additional questions concerning the bacterial fauna in hard ticks and will prompt researchers to perform detailed studies for better understanding of ixodid ticks associated bacteria.},
  keywords = {{T}icks ; {A}mblyomma ; {R}hipicephalus ; {B}orrelia species ; {D}omestic animals ; {O}romia ; {E}thiopia ; {ETHIOPIE}},
  booktitle = {},
  journal = {{T}icks and {T}ick-{B}orne {D}iseases},
  volume = {6},
  numero = {3},
  pages = {401--407},
  ISSN = {1877-959{X}},
  year = {2015},
  DOI = {10.1016/j.ttbdis.2015.03.009},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00013206},
}