@article{PAR00013079,
  title = {{I}nsights into bacterial colonization of intensive care patients' skin : the effect of chlorhexidine daily bathing},
  author = {{C}assir, {N}. and {P}apazian, {L}. and {F}ournier, {P}. {E}. and {R}aoult, {D}idier and {L}a {S}cola, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}kin is a major reservoir of bacterial pathogens in intensive care unit ({ICU}) patients. {T}he aim of this study was to assess the skin bacterial richness and diversity in {ICU} patients and the effect of {CHG} daily bathing on skin microbiota. {T}wenty {ICU} patients were included during an interventional period with {CHG} daily bathing (n = 10) and a control period (n = 10). {A}t day seven of hospitalization, eight skin swab samples (nares, axillary vaults, inguinal creases, manubrium and back) were taken from each patient. {T}he bacterial identification was performed by microbial culturomics. {W}e used the {S}hannon index to compare the diversity. {W}e obtained 5,000 colonies that yielded 61 bacterial species (9.15 +/- 3.7 per patient), including 15 (24.5 %) that had never been cultured from non-pathological human skin before, and three (4.9 %) that had never been cultured from human samples before. {N}otably, {G}ram-negative bacteria were isolated from all sites. {I}n the water-and-soap group, there was a higher risk of colonization with {G}ram-negative bacteria ({OR} = 6.05, 95 % {CI} [1.67-21.90]; {P} = 0.006). {I}n the {CHG} group, we observed more patients colonized by sporulating bacteria (9/10 vs. 3/10; {P} = 0.019) with a reduced skin bacterial richness ({P} = 0.004) and lower diversity (0.37, 95 % {CI} [0.33; 0.42] vs. 0.50, 95 % {CI} [0.48; 0.52]). {G}ram-negative bacteria are frequent and disseminated components of the transient skin flora in {ICU} patients. {CHG} daily bathing is associated with a reduction in {G}ram-negative bacteria colonization together with substantial skin microbiota shifts.},
  keywords = {},
  booktitle = {},
  journal = {{E}uropean {J}ournal of {C}linical {M}icrobiology & {I}nfectious {D}iseases},
  volume = {34},
  numero = {5},
  pages = {999--1004},
  ISSN = {0934-9723},
  year = {2015},
  DOI = {10.1007/s10096-015-2316-y},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00013079},
}