@article{PAR00013077,
  title = {{T}he aerobic activity of metronidazole against anaerobic bacteria},
  author = {{D}ione, {N}. and {K}helaifia, {S}. and {L}agier, {J}. {C}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ecently, the aerobic growth of strictly anaerobic bacteria was demonstrated using antioxidants. {M}etronidazole is frequently used to treat infections caused by anaerobic bacteria; however, to date its antibacterial activity was only tested in anaerobic conditions. {H}ere we aerobically tested using antioxidants the in vitro activities of metronidazole, gentamicin, doxycycline and imipenem against 10 common anaerobic and aerobic bacteria. {I}n vitro susceptibility testing was performed by the disk diffusion method, and minimum inhibitory concentrations ({MIC}s) were determined by {E}test. {A}erobic culture of the bacteria was performed at 37 degrees {C} using {S}chaedler agar medium supplemented with 1 mg/m{L} ascorbic acid and 0.1 mg/m{L} glutathione; the p{H} was adjusted to 7.2 by {IOM} {KOH}. {G}rowth of anaerobic bacteria cultured aerobically using antioxidants was inhibited by metronidazole after 72 h of incubation at 37 degrees {C}, with a mean inhibition diameter of 37.76 mm and an {MIC} of 1 mu g/m{L}; however, strains remained non-sensitive to gentamicin. {N}o growth inhibition of aerobic bacteria was observed after 24 h of incubation at 37 degrees {C} with metronidazole; however, inhibition was observed with doxycycline and imipenem used as controls. {T}hese results indicate that bacterial sensitivity to metronidazole is not related to the oxygen tension but is a result of the sensitivity of the micro-organism. {I}n future, both culture and antibiotic susceptibility testing of strictly anaerobic bacteria will be performed in an aerobic atmosphere using antioxidants in clinical microbiology laboratories.},
  keywords = {{M}etronidazole ; {A}ntioxidants ; {A}erobic susceptibility testing ; {I}n vitro ; activity ; {MALDI}-{TOF}/{MS}},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {A}ntimicrobial {A}gents},
  volume = {45},
  numero = {5},
  pages = {537--540},
  ISSN = {0924-8579},
  year = {2015},
  DOI = {10.1016/j.ijantimicag.2014.12.032},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00013077},
}