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Dione N., Khelaifia S., Lagier J. C., Raoult Didier. (2015). The aerobic activity of metronidazole against anaerobic bacteria. International Journal of Antimicrobial Agents, 45 (5), p. 537-540. ISSN 0924-8579.

Titre du document
The aerobic activity of metronidazole against anaerobic bacteria
Année de publication
2015
Type de document
Article référencé dans le Web of Science WOS:000353377800016
Auteurs
Dione N., Khelaifia S., Lagier J. C., Raoult Didier
Source
International Journal of Antimicrobial Agents, 2015, 45 (5), p. 537-540 ISSN 0924-8579
Recently, the aerobic growth of strictly anaerobic bacteria was demonstrated using antioxidants. Metronidazole is frequently used to treat infections caused by anaerobic bacteria; however, to date its antibacterial activity was only tested in anaerobic conditions. Here we aerobically tested using antioxidants the in vitro activities of metronidazole, gentamicin, doxycycline and imipenem against 10 common anaerobic and aerobic bacteria. In vitro susceptibility testing was performed by the disk diffusion method, and minimum inhibitory concentrations (MICs) were determined by Etest. Aerobic culture of the bacteria was performed at 37 degrees C using Schaedler agar medium supplemented with 1 mg/mL ascorbic acid and 0.1 mg/mL glutathione; the pH was adjusted to 7.2 by IOM KOH. Growth of anaerobic bacteria cultured aerobically using antioxidants was inhibited by metronidazole after 72 h of incubation at 37 degrees C, with a mean inhibition diameter of 37.76 mm and an MIC of 1 mu g/mL; however, strains remained non-sensitive to gentamicin. No growth inhibition of aerobic bacteria was observed after 24 h of incubation at 37 degrees C with metronidazole; however, inhibition was observed with doxycycline and imipenem used as controls. These results indicate that bacterial sensitivity to metronidazole is not related to the oxygen tension but is a result of the sensitivity of the micro-organism. In future, both culture and antibiotic susceptibility testing of strictly anaerobic bacteria will be performed in an aerobic atmosphere using antioxidants in clinical microbiology laboratories.
Plan de classement
Santé : généralités [050] ; Biotechnologies [084]
Identifiant IRD
PAR00013077
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