@article{PAR00013068,
  title = {{I}solation of {V}ermamoeba vermiformis and associated bacteria in hospital water},
  author = {{P}agnier, {I}. and {V}alles, {C}. and {R}aoult, {D}idier and {L}a {S}cola, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}o detect new potential pathogens in hospital water, we isolated free-living amoebae in water samples taken from three different hospitals in {M}arseille ({F}rance). {T}he samples were inoculated in media containing saline buffer and various bacteria as nutrient sources. {T}he isolated amoebae were identified by gene sequencing. {A}mong the 105 water samples, taken from 19 sites, we isolated 14 amoebae, of which 9 {V}ermamoeba vermiformis and 5 {A}canthamoeba sp. {N}one of the amoebae showed the presence of obligate bacterial endosymbionts. {B}ecause {V}. vermiformis was most commonly isolated, we used an axenic collection strain to isolate amoeba-resistant bacteria from the same sites. {T}he isolated bacterial species included {S}tenotrophomonas maltophilia and {L}egionella sp. {L}egionella taurinensis was isolated for the first time in association with amoebae. {A} strict intracellular bacterium was isolated, that may represent a new genus among the {C}hlamydiales. {W}e propose that it be named "{C}andidatus {R}ubidus massiliensis". {O}ur study shows that the isolation and identification of new pathogens associated with amoebae, which were previously performed using {A}canthamoeba sp., should instead use {V} vermiformis because this organism is more commonly associated with humans and is an essential complement of {A}canthamoeba sp. co-culture to study the ecology of hospital water supplies.},
  keywords = {{V}ermamoeba vermiformis ; {A}moeba resisting bacteria ; {C}hlamydiales ; {FRANCE}},
  booktitle = {},
  journal = {{M}icrobial {P}athogenesis},
  volume = {80},
  numero = {},
  pages = {14--20},
  ISSN = {0882-4010},
  year = {2015},
  DOI = {10.1016/j.micpath.2015.02.006},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00013068},
}