@article{PAR00012938,
  title = {{H}igh {A}topobium vaginae and {G}ardnerella vaginalis vaginal loads are associated with preterm birth},
  author = {{B}retelle, {F}. and {R}ozenberg, {P}. and {P}ascal, {A}. and {F}avre, {R}. and {B}ohec, {C}. and {L}oundou, {A}. and {S}enat, {M}. {V}. and {A}issi, {G}. and {L}esavre, {N}. and {B}runet, {J}. and {H}eckenroth, {H}. and {L}uton, {D}. and {R}aoult, {D}idier and {F}enollar, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground. {B}acterial vaginosis is a risk factor for preterm birth. {T}he various conventional methods for its diagnosis are laborious and not easily reproducible. {M}olecular quantification methods have been reported recently, but the specific risk factors they might identify remain unclear. {M}ethods. {A} prospective multicenter national study included pregnant women at risk of preterm birth. {A} quantitative molecular tool using a specific real-time polymerase chain reaction assay and serial dilutions of a plasmid suspension quantified {A}topobium vaginae, {G}ardnerella vaginalis, lactobacilli, {M}ycoplasma hominis, and the human albumin gene (for quality control). {R}esults. {I}n 813 pregnancies, high vaginal loads of either or both of {A}. vaginae and {G}. vaginalis were associated with preterm birth (hazard ratio [{HR}], 3.9; 95% confidence interval {{CI}}, 1.1-14.1; {P} = .031). {A} high vaginal load of {A}. vaginae was significantly associated with shortened time to delivery and therefore pregnancy length. {T}hese times were, respectively, 152.2 and 188.2 days ({HR}, 5.6; 95% {CI}, 1.5-21.3; {P} < .001) before 22 weeks, 149.0 and 183.2 days ({HR}, 2.8; 95% {CI}, 1.1-8.2; {P} = .048) before 28 weeks, and 132.6 and 170.4 days ({HR}, 2.2; 95% {CI}, 1.1-4.6; {P} = .033) before 32 weeks. {A}fter multivariate analysis, {A}. vaginae levels = 108 copies/m{L} remained significantly associated with delivery before 22 weeks of gestation (adjusted {HR}, 4.7; 95% {CI},.2-17.6; {P} = .014). {C}onclusions. {H}igh vaginal loads of {A}. vaginae and {G}. vaginalis are associated with late miscarriage and prematurity in high-risk pregnancies. {A} high vaginal load of {A}. vaginae ({DNA} level = 108 copies/m{L}) identifies a population at high risk of preterm birth. {F}urther studies that both screen for and then treat {A}. vaginae are needed.},
  keywords = {{A}topobium vaginae ; {G}ardnerella vaginalis ; molecular biology ; bacterial ; vaginosis ; preterm birth},
  booktitle = {},
  journal = {{C}linical {I}nfectious {D}iseases},
  volume = {60},
  numero = {6},
  pages = {860--867},
  ISSN = {1058-4838},
  year = {2015},
  DOI = {10.1093/cid/ciu966},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00012938},
}