@article{PAR00012926,
  title = {{C}omplementarity between targeted real-time specific {PCR} and conventional broad-range 16{S} r{DNA} {PCR} in the syndrome-driven diagnosis of infectious diseases},
  author = {{M}orel, {A}. {S}. and {D}ubourg, {G}. and {P}rudent, {E}. and {E}douard, {S}. and {G}ouriet, {F}. and {C}asalta, {J}. {P}. and {F}enollar, {F}. and {F}ournier, {P}. {E}. and {D}rancourt, {M}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{M}olecular tools have shown an added value in the diagnosis of infectious diseases, in particular for those caused by fastidious intracellular microorganisms, or in patients receiving antibiotics before sampling. {I}f 16{S} r{DNA} amplification had been gradually implemented in microbiology laboratories, specific real-time polymerase chain reaction ({PCR}) would have permitted an increase in the sensitivity of molecular methods and a reduction of contamination. {H}erein, we report our experience in the diagnosis of infectious diseases over two years, during which 32,948 clinical samples from 18,056 patients were received from {F}rance and abroad. {A}mong these samples, 81,476 {PCR}s were performed, of which 1,192 were positive. {M}olecular techniques detected intracellular microorganisms in 31.3 % of respiratory samples, 27.8 % of endocarditis samples and 51.9 % of adenitis samples. {E}xcluding intracellular bacteria, 25 % of the positive samples in this series were sterile in culture. {C}onventional broad-range {PCR} permitted the identification of fastidious and anaerobic microorganisms, but specific real-time {PCR} showed a significant superiority in the diagnosis of osteoarticular infections, in particular for those caused by {K}ingella kingae and {S}taphylococcus aureus, and for endocarditis diagnosis, specifically when {S}treptococcus gallolyticus and {S}taphylococcus aureus were involved. {T}he sensitivity of conventional broad-range {PCR} was 62.9 % concerning overall diagnoses for which both techniques had been performed. {T}hese findings should lead microbiologists to focus on targeted specific real-time {PCR} regarding the clinical syndrome. {F}inally, syndrome-driven diagnosis, which consists of testing a panel of microorganisms commonly involved for each syndrome, permitted the establishment of 31 incidental diagnoses.},
  keywords = {},
  booktitle = {},
  journal = {{E}uropean {J}ournal of {C}linical {M}icrobiology and {I}nfectious {D}iseases},
  volume = {34},
  numero = {3},
  pages = {561--570},
  ISSN = {0934-9723},
  year = {2015},
  DOI = {10.1007/s10096-014-2263-z},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00012926},
}