@article{PAR00012717,
  title = {{A}ccurate identification of {C}ulicidae at aquatic developmental stages by {MALDI}-{TOF} {MS} profiling},
  author = {{D}ieme, {C}. and {Y}ssouf, {A}. and {V}ega-{R}ua, {A}. and {B}erenger, {J}. {M}. and {F}ailloux, {A}. {B}. and {R}aoult, {D}idier and {P}arola, {P}. and {A}lmeras, {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he identification of mosquito vectors is generally based on morphological criteria, but for aquatic stages, morphological characteristics may be missing, leading to incomplete or incorrect identification. {T}he high cost of molecular biology techniques requires the development of an alternative strategy. {I}n the last decade, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry ({MALDI}-{TOF} {MS}) profiling has proved to be efficient for arthropod identification at the species level. {M}ethods: {T}o investigate the usefulness of {MALDI}-{TOF} {MS} for the identification of mosquitoes at aquatic stages, optimizations of sample preparation, diet, body parts and storage conditions were tested. {P}rotein extracts of whole specimens from second larval stage to pupae were selected for the creation of a reference spectra database. {T}he database included a total of 95 laboratory-reared specimens of 6 mosquito species, including {A}nopheles gambiae ({S} form), {A}nopheles coluzzi ({M} form), {C}ulex pipiens pipiens, {C}ulex pipiens molestus, {A}edes aegypti and 2 colonies of {A}edes albopictus. {R}esults: {T}he present study revealed that whole specimens at aquatic stages produced reproducible and singular spectra according to the mosquito species. {M}oreover, {MS} protein profiles appeared weakly affected by the diet provided. {D}espite the low diversity of some {MS} profiles, notably for cryptic species, clustering analyses correctly classified all specimens tested at the species level followed by the clustering of early vs. late aquatic developmental stages. {D}iscriminant mass peaks were recorded for the 6 mosquito species analyzed at larval stage 3 and the pupal stage. {Q}uerying against the reference spectra database of 149 new specimens at different aquatic stages from the 6 mosquito species revealed that 147 specimens were correctly identified at the species level and that early and late developmental stages were also distinguished. {C}onclusions: {T}he present work highlights that {MALDI}-{TOF} {MS} profiling may be useful for the rapid and reliable identification of mosquito species at aquatic stages. {W}ith this proteomic tool, it becomes now conceivable to survey mosquito breeding sites prior to the mosquitoes' emergence and to adapt anti-vectorial measures according to the mosquito fauna detected.},
  keywords = {{MALDI}-{TOF} mass spectrometry ; {C}ulicidae ; {A}quatic stages ; {S}pecies identification ; {V}ectors},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {7},
  numero = {},
  pages = {art. 544 [14 ]},
  ISSN = {1756-3305},
  year = {2014},
  DOI = {10.1186/s13071-014-0544-0},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00012717},
}