@article{PAR00012715, title = {{I}nfection of sand flies collected from different bio-geographical areas of {T}unisia with phleboviruses}, author = {{F}ares, {W}. and {C}harrel, {R}. {N}. and {D}achraoui, {K}. and {B}ichaud, {L}. and {B}arhoumi, {W}. and {D}erbali, {M}. and {C}herni, {S}. and {C}helbi, {I}. and de {L}amballerie, {X}avier and {Z}hioua, {E}.}, editor = {}, language = {{ENG}}, abstract = {{A}n entomological investigation performed in 2013 covering different bio-geographical areas varying from humid in the north to the arid in the center showed that sand flies of the subgenus {L}arroussius including {P}hlebotomus perniciosus, {P}hlebotomus perfiliewi, and {P}hlebotomus longicuspis are abundant and widely distributed in {T}unisia. {A} total of 3992 collected and pooled with up to 30 specimens per pool based on sex, trapping location and collection data were tested for the presence of phleboviruses by nested reverse transcriptase polymerase chain reaction and sequencing. {O}f a total of 135 pools, 23 were positive, yielding and minimum infection rate of 0.6%. {P}hylogenetic analysis performed using partial amino acid sequence in the polymerase gene showed that all these phleboviruses were grouped in one cluster clearly distinct from but closely related to {M}assilia virus and {G}ranada virus. {T}his putative novel virus, tentatively called {S}addaguia virus ({SADV}), is widely distributed in {T}unisia. {T}ogether with {T}oscana, {P}unique, and {U}tique viruses, {SADV} is the fourth recognized phlebovirus to be transmitted by sand flies in {T}unisia. {T}he medical and public health interest of {SADV} remains to be investigated.}, keywords = {{S}and flies ; {P}hlebovirus ; {S}addaguia virus ; {P}hlebotomus ; {E}merging ; {M}editerranean basin ; {TUNISIE}}, booktitle = {}, journal = {{A}cta {T}ropica}, volume = {141}, numero = {{A}}, pages = {1--6}, ISSN = {0001-706{X}}, year = {2015}, DOI = {10.1016/j.actatropica.2014.09.009}, URL = {https://www.documentation.ird.fr/hor/{PAR}00012715}, }