@article{PAR00012708,
  title = {{R}eal-time video imaging as a new and rapid tool for antibiotic susceptibility testing by the disc diffusion method : a paradigm for evaluating resistance to imipenem and identifying extended-spectrum beta-lactamases},
  author = {{L}e {P}age, {S}. and {R}aoult, {D}idier and {R}olain, {J}. {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he disc diffusion method has long been considered the standard technique for antibiotic susceptibility testing ({AST}) in clinical microbiology laboratories because of its simplicity, reproducibility and low cost compared with commercial automated microdilution systems that are usually more rapid but less sensitive for detecting important mechanisms of resistance. {H}ere we measured reading zone diameters around antibiotics in a series of 25 well-characterised {G}ram-negative bacteria by the disc diffusion technique in real-time using an {A}dvencis {B}io-{S}ystem instrument consisting of a real-time high-resolution video imager in a dedicated incubator. {T}he susceptibility of wild-type {G}ram-negative bacteria to imipenem, determined by reading the diameter of inhibition, was detectable as early as 3.5 h (mean time 3.7 +/- 0.45 h), whereas carbapenemase-producing {G}ram-negative bacteria could be correctly categorised as early as 3 h (mean time 4.2 +/- 0.8 h) of incubation. {S}imilarly, the characteristic champagne cork aspect of extended-spectrum p-lactamase ({ESBL}) could be detected by the system as early as 3.5 h. {M}oreover, we present here for the first time video movies of the appearance of the diameter of inhibition by disc diffusion in real-time. {T}his preliminary study using a new and innovative technology provides for a renewed interest for microbiologists who wish to continue to use the disc diffusion method as a reference method for {AST}. {N}ew video imaging technology presents a proof of concept that could improve the real-time management of patients with {AST} within a very rapid turnaround time and can provide a large financial saving for hospitals.},
  keywords = {{V}ideo imaging ; {C}arbapenemase ; {A}ntibiotic resistance ; {A}ntibiotic susceptibility},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {A}ntimicrobial {A}gents},
  volume = {45},
  numero = {1},
  pages = {61--65},
  ISSN = {0924-8579},
  year = {2015},
  DOI = {10.1016/j.ijantimicag.2014.08.014},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00012708},
}