Publications des scientifiques de l'IRD

Ngouana T. K., Krasteva D., Drakulovski P., Toghueo R. K., Kouanfack C., Ambe A., Reynes J., Delaporte Eric, Boyom F. F., Mallie M., Bertout S. (2015). Investigation of minor species Candida africana, Candida stellatoidea and Candida dubliniensis in the Candida albicans complex among Yaounde (Cameroon) HIV-infected patients. Mycoses, 58 (1), p. 33-39. ISSN 0933-7407.

Titre du document
Investigation of minor species Candida africana, Candida stellatoidea and Candida dubliniensis in the Candida albicans complex among Yaounde (Cameroon) HIV-infected patients
Année de publication
2015
Type de document
Article référencé dans le Web of Science WOS:000346726700006
Auteurs
Ngouana T. K., Krasteva D., Drakulovski P., Toghueo R. K., Kouanfack C., Ambe A., Reynes J., Delaporte Eric, Boyom F. F., Mallie M., Bertout S.
Source
Mycoses, 2015, 58 (1), p. 33-39 ISSN 0933-7407
Minor species of the Candida albicans complex may cause overestimation of the epidemiology of C. albicans, and misidentifications could mask their implication in human pathology. Authors determined the occurrence of minor species of the C. albicans complex (C. africana, C. dubliniensis and C. stellatoidea) among Yaounde HIV-infected patients, Cameroon. Stool, vaginal discharge, urine and oropharyngeal samples were analysed by mycological diagnosis. Isolates were identified by conventional methods and mass spectrometry (MS; carried out by the matrix-assisted laser desorption-ionisation time-of-flight MS protocol). Candida albicans isolates were thereafter submitted to the PCR amplification of the Hwp1 gene. The susceptibility of isolates to antifungal drugs was tested using the Clinical and Laboratory Standards Institute M27-A3 protocol. From 115 C. albicans obtained isolates, neither C. dubliniensis nor C. stellatoidea was observed; two strains of C. africana (422PV and 448PV) were identified by PCR electrophoretic profiles at 700bp. These two C. africana strains were vaginal isolates. The isolate 448PV was resistant to ketoconazole at the minimal inhibitory concentration of 2g ml(-1), and showed reduced susceptibility to amphotericin B at 1g ml(-1). This first report on C. africana occurrence in Cameroon brings clues for the understanding of the global epidemiology of this yeast as well as that of minor species of the C. albicans complex.
Plan de classement
Santé : généralités [050] ; Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
CAMEROUN
Localisation
Fonds IRD
Identifiant IRD
PAR00012706
Contact