@article{PAR00012574,
  title = {{W}orldwide emergence of colistin resistance in {K}lebsiella pneumoniae from healthy humans and patients in {L}ao {PDR}, {T}hailand, {I}srael, {N}igeria and {F}rance owing to inactivation of the {P}ho{P}/{P}ho{Q} regulator mgr{B} : an epidemiological and molecular study},
  author = {{O}laitan, {A}. {O}. and {D}iene, {S}. {M}. and {K}empf, {M}. and {B}errazeg, {M}. and {B}akour, {S}. and {G}upta, {S}. {K}. and {T}hongmalayvong, {B}. and {A}kkhavong, {K}. and {S}omphavong, {S}. and {P}aboriboune, {P}. and {C}haisiri, {K}. and {K}omalamisra, {C}. and {A}delowo, {O}. {O}. and {F}agade, {O}. {E}. and {B}anjo, {O}. {A}. and {O}ke, {A}. {J}. and {A}dler, {A}. and {A}ssous, {M}. {V}. and {M}orand, {S}. and {R}aoult, {D}idier and {R}olain, {J}. {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he emergence of colistin-resistant {K}lebsiella pneumoniae ({CRKP}) is a major public health concern worldwide. {I}n this study, the prevalence and molecular basis of colistin resistance in {CRKP} isolated from healthy individuals and patients in {L}ao {PDR}, {T}hailand, {N}igeria and {F}rance were investigated. {S}tool samples were screened by culture for the presence of colistin-resistant {K}lebsiella spp. {W}hole-genome sequence analysis was used to decipher the molecular mechanism of colistin resistance in a bla({NDM}-1)-positive in vitro-selected {CRKP} mutant. {PCR} amplification and sequencing of the mgr{B} genetic environment was performed for all {CRKP} isolates as well as control colistin-susceptible {K}. pneumoniae ({CSKP}) isolates recovered from the same stools. {A} total of 869 stool samples were screened for colistin-resistant {K}lebsiella spp., yielding 32 {CRKP} and 2 colistin-resistant {K}lebsiella oxytoca. {C}omparative whole-genome sequence analysis revealed that an in vitro-selected {CRKP} mutant had an insertion sequence in its mgr{B} gene, as well as missense mutations in other selected clones. {O}f the 34 colistin-resistant {K}lebsiella spp. isolates, 14 (41.2%; 13 {CRKP} and 1 {K}. oxytoca) from the four countries also had various defects in their mgr{B} genes, but no such defects were found in the {CSKP} controls ({P} < 10(-4)). {F}ew mutations were observed in pmr{AB} compared with mgr{B} among the {CRKP} isolates. {T}he worldwide emergence of {CRKP} is a major public health concern. {D}etection and surveillance of such strains are warranted to prevent an uncontrollable pandemic. {I}nactivation of the {P}ho{P}/{P}ho{Q} regulator gene mgr{B} is associated with >= 40% of colistin resistance among the {CRKP} isolates observed in this study.},
  keywords = {{A}ntibiotic resistance ; {T}ransposon ; {G}enome analysis ; {C}olistin resistance ; mgr{B} gene ; {T}wo-component systems ; {LAOS} ; {THAILANDE} ; {NIGERIA} ; {ISRAEL} ; {FRANCE}},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {A}ntimicrobial {A}gents},
  volume = {44},
  numero = {6},
  pages = {500--507},
  ISSN = {0924-8579},
  year = {2014},
  DOI = {10.1016/j.ijantimicag.2014.07.020},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00012574},
}