Olaitan A. O., Diene S. M., Kempf M., Berrazeg M., Bakour S., Gupta S. K., Thongmalayvong B., Akkhavong K., Somphavong S., Paboriboune P., Chaisiri K., Komalamisra C., Adelowo O. O., Fagade O. E., Banjo O. A., Oke A. J., Adler A., Assous M. V., Morand S., Raoult Didier, Rolain J. M. (2014). Worldwide emergence of colistin resistance in Klebsiella pneumoniae from healthy humans and patients in Lao PDR, Thailand, Israel, Nigeria and France owing to inactivation of the PhoP/PhoQ regulator mgrB : an epidemiological and molecular study. International Journal of Antimicrobial Agents, 44 (6), p. 500-507. ISSN 0924-8579.
Titre du document
Worldwide emergence of colistin resistance in Klebsiella pneumoniae from healthy humans and patients in Lao PDR, Thailand, Israel, Nigeria and France owing to inactivation of the PhoP/PhoQ regulator mgrB : an epidemiological and molecular study
Année de publication
2014
Auteurs
Olaitan A. O., Diene S. M., Kempf M., Berrazeg M., Bakour S., Gupta S. K., Thongmalayvong B., Akkhavong K., Somphavong S., Paboriboune P., Chaisiri K., Komalamisra C., Adelowo O. O., Fagade O. E., Banjo O. A., Oke A. J., Adler A., Assous M. V., Morand S., Raoult Didier, Rolain J. M.
Source
International Journal of Antimicrobial Agents, 2014,
44 (6), p. 500-507 ISSN 0924-8579
The emergence of colistin-resistant Klebsiella pneumoniae (CRKP) is a major public health concern worldwide. In this study, the prevalence and molecular basis of colistin resistance in CRKP isolated from healthy individuals and patients in Lao PDR, Thailand, Nigeria and France were investigated. Stool samples were screened by culture for the presence of colistin-resistant Klebsiella spp. Whole-genome sequence analysis was used to decipher the molecular mechanism of colistin resistance in a bla(NDM-1)-positive in vitro-selected CRKP mutant. PCR amplification and sequencing of the mgrB genetic environment was performed for all CRKP isolates as well as control colistin-susceptible K. pneumoniae (CSKP) isolates recovered from the same stools. A total of 869 stool samples were screened for colistin-resistant Klebsiella spp., yielding 32 CRKP and 2 colistin-resistant Klebsiella oxytoca. Comparative whole-genome sequence analysis revealed that an in vitro-selected CRKP mutant had an insertion sequence in its mgrB gene, as well as missense mutations in other selected clones. Of the 34 colistin-resistant Klebsiella spp. isolates, 14 (41.2%; 13 CRKP and 1 K. oxytoca) from the four countries also had various defects in their mgrB genes, but no such defects were found in the CSKP controls (P < 10(-4)). Few mutations were observed in pmrAB compared with mgrB among the CRKP isolates. The worldwide emergence of CRKP is a major public health concern. Detection and surveillance of such strains are warranted to prevent an uncontrollable pandemic. Inactivation of the PhoP/PhoQ regulator gene mgrB is associated with >= 40% of colistin resistance among the CRKP isolates observed in this study.
Plan de classement
Santé : généralités [050]
;
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
LAOS ; THAILANDE ; NIGERIA ; ISRAEL ; FRANCE
Identifiant IRD
PAR00012574
