%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Aubry, F.
%A Nougairede, A.
%A de Fabritus, L.
%A Querat, G.
%A Gould, E. A.
%A de Lamballerie, Xavier
%T Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons
%D 2014
%L PAR00012468
%G ENG
%J Journal of General Virology
%@ 0022-1317
%M ISI:000345309600013
%N 11
%P 2462-2467
%R 10.1099/vir.0.068023-0
%U https://www.documentation.ird.fr/hor/PAR00012468
%V 95
%W Horizon (IRD)
%X Reverse genetics is a key methodology for producing genetically modified RNA viruses and deciphering cellular and viral biological properties, but methods based on the preparation of plasmid-based complete viral genomes are laborious and unpredictable. Here, both wild-type and genetically modified infectious RNA viruses were generated in days using the newly described ISA (infectious-subgenomic-amplicons) method. This new versatile and simple procedure may enhance our capacity to obtain infectious RNA viruses from PCR-amplified genetic material.
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