@article{PAR00012468,
  title = {{S}ingle-stranded positive-sense {RNA} viruses generated in days using infectious subgenomic amplicons},
  author = {{A}ubry, {F}. and {N}ougairede, {A}. and de {F}abritus, {L}. and {Q}uerat, {G}. and {G}ould, {E}. {A}. and de {L}amballerie, {X}avier},
  editor = {},
  language = {{ENG}},
  abstract = {{R}everse genetics is a key methodology for producing genetically modified {RNA} viruses and deciphering cellular and viral biological properties, but methods based on the preparation of plasmid-based complete viral genomes are laborious and unpredictable. {H}ere, both wild-type and genetically modified infectious {RNA} viruses were generated in days using the newly described {ISA} (infectious-subgenomic-amplicons) method. {T}his new versatile and simple procedure may enhance our capacity to obtain infectious {RNA} viruses from {PCR}-amplified genetic material.},
  keywords = {},
  booktitle = {},
  journal = {{J}ournal of {G}eneral {V}irology},
  volume = {95},
  numero = {11},
  pages = {2462--2467},
  ISSN = {0022-1317},
  year = {2014},
  DOI = {10.1099/vir.0.068023-0},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00012468},
}