Aubry F., Nougairede A., de Fabritus L., Querat G., Gould E. A., de Lamballerie Xavier. (2014). Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons. Journal of General Virology, 95 (11), p. 2462-2467. ISSN 0022-1317.
Titre du document
Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons
Aubry F., Nougairede A., de Fabritus L., Querat G., Gould E. A., de Lamballerie Xavier
Source
Journal of General Virology, 2014,
95 (11), p. 2462-2467 ISSN 0022-1317
Reverse genetics is a key methodology for producing genetically modified RNA viruses and deciphering cellular and viral biological properties, but methods based on the preparation of plasmid-based complete viral genomes are laborious and unpredictable. Here, both wild-type and genetically modified infectious RNA viruses were generated in days using the newly described ISA (infectious-subgenomic-amplicons) method. This new versatile and simple procedure may enhance our capacity to obtain infectious RNA viruses from PCR-amplified genetic material.
