%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Mishra, A. K.
%A Dufour, H.
%A Roche, P. H.
%A Lonjon, M.
%A Raoult, Didier
%A Fournier, P. E.
%T Molecular revolution in the diagnosis of microbial brain abscesses
%D 2014
%L PAR00012460
%G ENG
%J European Journal of Clinical Microbiology and Infectious Diseases
%@ 0934-9723
%M ISI:000345138300001
%N 12
%P 2083-2093
%R 10.1007/s10096-014-2166-z
%U https://www.documentation.ird.fr/hor/PAR00012460
%V 33
%W Horizon (IRD)
%X A brain abscess is a life-threatening infection, frequently with serious sequelae. Culture-based methods present many limitations and do not enable an exhaustive documentation of the bacterial flora. 16S rRNA-based amplification, cloning, and high-throughput sequencing have dramatically increased the number of identified agents of brain abscesses, showing that the causative flora is polymicrobial in up to 40 % of cases, with the presence of at least one anaerobic bacterium. In contrast, real-time polymerase chain reaction (PCR) technology is an appealing alternative to culture-based methods for diagnosing brain abscesses due to its speed, sensitivity, and specificity. Molecular typing is available for several bacterial and fungal genera, and this user-friendly tool is accessible for the clinical microbiology laboratory to diagnose microbes involved in a brain abscess. This article reviews the applications of the currently available tools for the etiological diagnosis of a brain abscess.
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