@article{PAR00012460,
  title = {{M}olecular revolution in the diagnosis of microbial brain abscesses},
  author = {{M}ishra, {A}. {K}. and {D}ufour, {H}. and {R}oche, {P}. {H}. and {L}onjon, {M}. and {R}aoult, {D}idier and {F}ournier, {P}. {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A} brain abscess is a life-threatening infection, frequently with serious sequelae. {C}ulture-based methods present many limitations and do not enable an exhaustive documentation of the bacterial flora. 16{S} r{RNA}-based amplification, cloning, and high-throughput sequencing have dramatically increased the number of identified agents of brain abscesses, showing that the causative flora is polymicrobial in up to 40 % of cases, with the presence of at least one anaerobic bacterium. {I}n contrast, real-time polymerase chain reaction ({PCR}) technology is an appealing alternative to culture-based methods for diagnosing brain abscesses due to its speed, sensitivity, and specificity. {M}olecular typing is available for several bacterial and fungal genera, and this user-friendly tool is accessible for the clinical microbiology laboratory to diagnose microbes involved in a brain abscess. {T}his article reviews the applications of the currently available tools for the etiological diagnosis of a brain abscess.},
  keywords = {},
  booktitle = {},
  journal = {{E}uropean {J}ournal of {C}linical {M}icrobiology and {I}nfectious {D}iseases},
  volume = {33},
  numero = {12},
  pages = {2083--2093},
  ISSN = {0934-9723},
  year = {2014},
  DOI = {10.1007/s10096-014-2166-z},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00012460},
}