Mishra A. K., Dufour H., Roche P. H., Lonjon M., Raoult Didier, Fournier P. E. (2014). Molecular revolution in the diagnosis of microbial brain abscesses. European Journal of Clinical Microbiology and Infectious Diseases, 33 (12), p. 2083-2093. ISSN 0934-9723.
Titre du document
Molecular revolution in the diagnosis of microbial brain abscesses
Mishra A. K., Dufour H., Roche P. H., Lonjon M., Raoult Didier, Fournier P. E.
Source
European Journal of Clinical Microbiology and Infectious Diseases, 2014,
33 (12), p. 2083-2093 ISSN 0934-9723
A brain abscess is a life-threatening infection, frequently with serious sequelae. Culture-based methods present many limitations and do not enable an exhaustive documentation of the bacterial flora. 16S rRNA-based amplification, cloning, and high-throughput sequencing have dramatically increased the number of identified agents of brain abscesses, showing that the causative flora is polymicrobial in up to 40 % of cases, with the presence of at least one anaerobic bacterium. In contrast, real-time polymerase chain reaction (PCR) technology is an appealing alternative to culture-based methods for diagnosing brain abscesses due to its speed, sensitivity, and specificity. Molecular typing is available for several bacterial and fungal genera, and this user-friendly tool is accessible for the clinical microbiology laboratory to diagnose microbes involved in a brain abscess. This article reviews the applications of the currently available tools for the etiological diagnosis of a brain abscess.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Santé : généralités [050]
