@article{PAR00012380,
  title = {{M}sp{A}-{M}ycobacterium tuberculosis-transformant with reduced virulence : the "unbirthday paradigm"},
  author = {{L}amrabet, {O}. and {G}higo, {E}. and {M}ege, {J}. {L}. and {L}epidi, {H}. and {N}appez, {C}. and {R}aoult, {D}idier and {D}rancourt, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{E}xpressing msp{A} porin gene from {M}ycobacterium smegmatis in {M}ycobacterium tuberculosis attenuated this pathogen. {I}ntracellular growth of the transformants into free-living amoeba and murine and human macrophages decreased. {F}urthermore, transformants decreased the microbicidal program of human monocyte-derived macrophages. {BALB}/c mice inoculated with transformants exhibited higher weights, lower histological lesions and lower {M}. tuberculosis inoculum in the liver, spleen and lungs than control mice challenged with wild-type {M}. tuberculosis. {P}reliminary evaluation indicated that mice inoculated with this transformant showed higher weights and lower numbers of lung nodules and tissular mycobacteria than control mice when challenged with wild-type {M}. tuberculosis. {S}imilar to the paradoxical "unbirthday" gift coined by {L}ewis {C}arroll in {A}lice's {A}dventures in {W}onderland, adding msp{A} gene reduced the virulence of {M}. tuberculosis and yielded a protective effect. {L}ost of non-virulence genes is a mechanism for virulence in mycobacteria. {E}ngineering non-virulence genes in {M}. tuberculosis may yield strains with decreased virulence and increased immunogenicity.},
  keywords = {{M}ycobacterium tuberculosis ; {V}irulence ; {V}accine ; {P}orin ; {M}sp{A} ; {M}ycobacterium smegmatis},
  booktitle = {},
  journal = {{M}icrobial {P}athogenesis},
  volume = {76},
  numero = {},
  pages = {10--18},
  ISSN = {0882-4010},
  year = {2014},
  DOI = {10.1016/j.micpath.2014.08.003},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00012380},
}