%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Sharma, V.
%A Colson, P.
%A Giorgi, R.
%A Pontarotti, P.
%A Raoult, Didier
%T DNA-dependent RNA polymerase detects hidden giant viruses in published databanks
%D 2014
%L PAR00012373
%G ENG
%J Genome Biology and Evolution
%@ 1759-6653
%K DNA-dependent RNA polymerase ; giant virus ; "Megavirales" ; Mimivirus ; dark matter ; metagenomes ; domains of life ; environment
%M ISI:000342583200006
%N 7
%P 1603-1610
%R 10.1093/gbe/evu128
%U https://www.documentation.ird.fr/hor/PAR00012373
%V 6
%W Horizon (IRD)
%X Environmental metagenomic studies show that there is a "darkmatter," composed of sequences not linked to any known organism, as determined mainly using ribosomal DNA (rDNA) sequences, which therefore ignore giant viruses. DNA-dependent RNA polymerase (RNAP) genes are universal in microbes and conserved in giant viruses and may replace rDNA for identifying microbes. We found while reconstructing RNAP subunit 2 (RNAP2) phylogeny that a giant virus sequenced together with the genome of a large eukaryote, Hydra magnipapillata, has been overlooked. To explore the dark matter, we used viral RNAP2 and reconstructed putative ancestral RNAP2, which were significantly superior in detecting distant clades than current sequences, and we revealed two additional unknown mimiviruses, misclassified as an euryarchaeote and an oomycete plant pathogen, and detected unknown putative viral clades. We suggest using RNAP systematically to decipher the black matter and identify giant viruses.
%$ 052 ; 020