@article{PAR00012373,
  title = {{DNA}-dependent {RNA} polymerase detects hidden giant viruses in published databanks},
  author = {{S}harma, {V}. and {C}olson, {P}. and {G}iorgi, {R}. and {P}ontarotti, {P}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{E}nvironmental metagenomic studies show that there is a "darkmatter," composed of sequences not linked to any known organism, as determined mainly using ribosomal {DNA} (r{DNA}) sequences, which therefore ignore giant viruses. {DNA}-dependent {RNA} polymerase ({RNAP}) genes are universal in microbes and conserved in giant viruses and may replace r{DNA} for identifying microbes. {W}e found while reconstructing {RNAP} subunit 2 ({RNAP}2) phylogeny that a giant virus sequenced together with the genome of a large eukaryote, {H}ydra magnipapillata, has been overlooked. {T}o explore the dark matter, we used viral {RNAP}2 and reconstructed putative ancestral {RNAP}2, which were significantly superior in detecting distant clades than current sequences, and we revealed two additional unknown mimiviruses, misclassified as an euryarchaeote and an oomycete plant pathogen, and detected unknown putative viral clades. {W}e suggest using {RNAP} systematically to decipher the black matter and identify giant viruses.},
  keywords = {{DNA}-dependent {RNA} polymerase ; giant virus ; "{M}egavirales" ; {M}imivirus ; dark matter ; metagenomes ; domains of life ; environment},
  booktitle = {},
  journal = {{G}enome {B}iology and {E}volution},
  volume = {6},
  numero = {7},
  pages = {1603--1610},
  ISSN = {1759-6653},
  year = {2014},
  DOI = {10.1093/gbe/evu128},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00012373},
}