@article{PAR00012307, title = {{E}ukaryote culturomics of the gut reveals new species}, author = {{G}ouba, {N}. and {R}aoult, {D}idier and {D}rancourt, {M}.}, editor = {}, language = {{ENG}}, abstract = {{T}he repertoire of microeukaryotes in the human gut has been poorly explored, mainly in individuals living in northern hemisphere countries. {W}e further explored this repertoire using {PCR}-sequencing and culture in seven individuals living in four tropical countries. {A} total of 41 microeukaryotes including 38 different fungal species and three protists were detected. {F}our fungal species, {D}avidiella tassiana, {D}avidiella sp., {C}orticiaceae sp., and {P}enicillium sp., were uniquely detected by culture; 27 fungal species were uniquely detected using {PCR}-sequencing and {C}andida albicans, {C}andida glabrata, {T}richosporon asahii, {C}lavispora lusitaniae, {D}ebaryomyces hansenii, {M}alassezia restricta, and {M}alassezia sp. were detected using both molecular and culture methods. {F}ourteen microeukaryotes were shared by the seven individuals, whereas 27 species were found in only one individual, including 11 species in {A}mazonia, nine species in {P}olynesia, five species in {I}ndia, and two species in {S}enegal. {T}hese data support a worldwide distribution of {M}alassezia sp., {T}richosporon sp., and {C}andida sp. in the gut mycobiome. {H}ere, 13 fungal species and two protists, {S}tentor roeseli and {V}orticella campanula, were observed for first time in the human gut. {T}his study revealed a previously unsuspected diversity in the repertoire of human gut microeukaryotes, suggesting spots for further exploring this repertoire.}, keywords = {}, booktitle = {}, journal = {{P}los {O}ne}, volume = {9}, numero = {9}, pages = {e106994}, ISSN = {1932-6203}, year = {2014}, DOI = {10.1371/journal.pone.0106994}, URL = {https://www.documentation.ird.fr/hor/{PAR}00012307}, }