Chabriere E., Gonzalez D., Azza S., Durand P., Shiekh F. A., Moal V., Baudoin J. P., Pagnier I., Raoult Didier. (2014). Fetuin is the key for nanon self-propagation. Microbial Pathogenesis, 73, p. 25-30. ISSN 0882-4010.
Chabriere E., Gonzalez D., Azza S., Durand P., Shiekh F. A., Moal V., Baudoin J. P., Pagnier I., Raoult Didier
Source
Microbial Pathogenesis, 2014,
73, p. 25-30 ISSN 0882-4010
"Nanobacteria", also known as nanons or calciprotein particles (CPP), are nano-sized protein mineral complexes which have been isolated from numerous biological sources. Nanons possess self-replication properties and contain only serum proteins (e.g. Fetuin-A, Albumin). Herein, we develop a simplified in vitro model of nanons propagation composed of only fetuin-A as a protein. Using this model, we demonstrate that fetuin from nanons possesses a different, non-native conformation. Moreover, we show that nanons induce soluble fetuin-A precipitation which could serve as a template for calcification. This phenomenon explains the observed self-propagating properties that mimic infectious behavior. We also demonstrate that renal calculi are capable of inducing a conformational change in fetuin-A, suggesting that the propagation phenomenon of nanons may occur in vivo.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Santé : généralités [050]
