@article{PAR00012019,
  title = {{A} polyphasic strategy incorporating genomic data for the taxonomic description of novel bacterial species},
  author = {{R}amasamy, {D}. and {M}ishra, {A}. {K}. and {L}agier, {J}. {C}. and {P}adhmanabhan, {R}. and {R}ossi, {M}. and {S}entausa, {E}. and {R}aoult, {D}idier and {F}ournier, {P}. {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}urrently, bacterial taxonomy relies on a polyphasic approach based on the combination of phenotypic and genotypic characteristics. {H}owever, the current situation is paradoxical in that the genetic criteria that are used, including {DNA}-{DNA} hybridization, 16{S} r{RNA} gene sequence nucleotide similarity and phylogeny, and {DNA} {G}+{C} content, have significant limitations, but genome sequences that contain the whole genetic information of bacterial strains are not used for taxonomic purposes, despite the decreasing costs of sequencing and the increasing number of available genomes. {R}ecently, we diversified bacterial culture conditions with the aim of isolating uncultivated bacteria. {T}o classify the putative novel species that we cultivated, we used a polyphasic strategy that included phenotypic as well as genomic criteria (genome characteristics as well as genomic sequence similarity). {H}erein, we review the pros and cons of genome sequencing for taxonomy and propose that the incorporation of genome sequences in taxonomic studies has the advantage of using reliable and reproducible data. {T}his strategy, which we name taxono-genomics, may contribute to the taxonomic classification of bacteria.},
  keywords = {},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {S}ystematic and {E}volutionary {M}icrobiology},
  volume = {64},
  numero = {2},
  pages = {384--391},
  ISSN = {1466-5026},
  year = {2014},
  DOI = {10.1099/ijs.0.057091-0},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00012019},
}