Yssouf A., Socolovschi C., Leulmi H., Kernif T., Bitam I., Audoly G., Almeras L., Raoult Didier, Parola P. (2014). Identification of flea species using MALDI-TOF/MS. Comparative Immunology Microbiology and Infectious Diseases, 37 (3), p. 153-157. ISSN 0147-9571.
Yssouf A., Socolovschi C., Leulmi H., Kernif T., Bitam I., Audoly G., Almeras L., Raoult Didier, Parola P.
Source
Comparative Immunology Microbiology and Infectious Diseases, 2014,
37 (3), p. 153-157 ISSN 0147-9571
In the present study, a molecular proteomics (MALDI-TOF/MS) approach was used as a tool for identifying flea vectors. We measured the MS spectra from 38 flea specimens of 5 species including Ctenocephalides felis, Ctenocephalides canis, Archaeopsylla erinacei, Xenopsylla cheopis and Stenoponia tripectinata. A blind test performed with 24 specimens from species included in a library spectral database confirmed that MALDI-TOF/MS is an effective tool for discriminating flea species. Although fresh and 70% ethanol-conserved samples subjected to MALDI-TOF/MS in blind tests were correctly classified, only MS spectra of quality from fresh specimens were sufficient for accurate and significant identification. A cluster analysis highlighted that the MALDI Biotyper can be used for studying the phylogeny of fleas.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Entomologie médicale / Parasitologie / Virologie [052]
