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Pumhom P., Pognon D., Yangtara S., Thaprathorn N., Milocco C., Douangboupha B., Herder Stéphane, Chaval Y., Morand S., Jittapalapong S., Desquesnes M. (2014). Molecular prevalence of Trypanosoma spp. in wild rodents of Southeast Asia : influence of human settlement habitat. Epidemiology and Infection, 142 (6), p. 1221-1230. ISSN 0950-2688.

Titre du document
Molecular prevalence of Trypanosoma spp. in wild rodents of Southeast Asia : influence of human settlement habitat
Année de publication
2014
Type de document
Article référencé dans le Web of Science WOS:000334247600012
Auteurs
Pumhom P., Pognon D., Yangtara S., Thaprathorn N., Milocco C., Douangboupha B., Herder Stéphane, Chaval Y., Morand S., Jittapalapong S., Desquesnes M.
Source
Epidemiology and Infection, 2014, 142 (6), p. 1221-1230 ISSN 0950-2688
This study investigated the molecular prevalence of Trypanosoma lewisi and T. evansi in wild rodents from Cambodia, Lao PDR and Thailand. Between 2008 and 2012, rodents (and shrews) were trapped in nine locations and 616 of these were tested using three sets of primers: TRYP1 (amplifying ITS1 of ribosomal DNA of all trypanosomes), TBR (amplifying satellite genomic DNA of Trypanozoon parasites) and LEW1 (amplifying ITS1 of ribosomal DNA of T. lewisi). Based on the size of the PCR products using TRYP1, 17% were positive for T. lewisi and 1 center dot 0% positive for Trypanozoon. Results were confirmed by sequencing PCR products and by using more specific primers (LEW1 and TBR). The specificity of TRYP1 primers, however, failed as rodent DNA was amplified in some instances, giving unexpected product sizes. Using LEW1 primers, 13 center dot 3% of the samples were confirmed positive for T. lewisi, both by PCR and sequencing. In Thailand, T. lewisi was found in Rattus tanezumi, R. exulans and Berylmys; in Lao PDR, in R. tanezumi and R. exulans, and in Cambodia in R. tanezumi, R. exulans and R. norvegicus. Using TBR, 1 center dot 3% of the samples tested positive for Trypanozoon by PCR and sequencing; T. evansi is the only species of the Trypanozoon subgenus possibly present in wild Asian rodents. These results confirmed its presence in rodents from Thailand (R. tanezumi), Lao PDR (R. tanezumi, R. nitidus) and Cambodia (R. tanezumi, Niviventer fulvescens, Maxomys surifer). Based on the information related to rodent trapping, it was found that rodent species trapped in and around human dwellings had a higher prevalence of T. lewisi infection. R. tanezumi and R. exulans, two synanthropic species, were mainly found infected in this habitat suggesting a role as a reservoir and thus a potential source of T. lewisi for human infection.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052] ; Sciences du monde animal [080]
Description Géographique
CAMBODGE ; LAOS ; THAILANDE
Localisation
Fonds IRD
Identifiant IRD
PAR00011675
Contact