@article{PAR00011617,
  title = {{C}linical significance of intra-host variability of {D}engue-1 virus in venous and capillary blood},
  author = {{D}escloux, {E}. and {L}a {F}uentez, {C}. and {R}oca, {Y}. and {D}e {L}amballerie, {X}avier},
  editor = {},
  language = {{ENG}},
  abstract = {{D}engue fever represents a major public health problem. {B}oth viral and host immune factors are involved in severe infections. {H}umans and mosquito-vectors are infected with diverse viral populations that may play a role in viral adaptation and disease pathogenesis. {O}ur objective was to analyse the intra-host genetic variability of dengue virus type 1 ({DENV}-1) in the venous and capillary blood and its relationships with the clinical presentation of dengue fever. {E}arly serum samples were collected in 2009 from ten {DENV}-1-infected patients hospitalized in {S}anta {C}ruz de la {S}ierra, {B}olivia. {P}artial viral envelope sequences were analysed at the inter-host and intra-host level. {F}or each patient, an average of 56 clone sequences was analysed both in the venous sector and the capillary sector (from right and left hands). {T}he ten consensus sequences were highly similar. {T}he intra-host {DENV}-1 genetic variability was significantly lower in the venous sector than in the capillary sector, and in patients with haemorrhagic symptoms than in those without haemorrhagic symptoms, particularly in capillary samples. {N}o relation was found with sex, age, dengue {I}g{G}-serological status, day of serum sampling, or viral load. {S}ignificant relationships were found between the clinical presentation of dengue fever and the variability of viral populations within hosts, particularly in capillary samples. {T}he observed variability of envelope sequences at the early phase of dengue infection was not critically influenced by the previous dengue serological status of patients. {A}n important part of viral microevolution may occur in the capillary sector and influence the mechanisms of severe forms.},
  keywords = {{C}apillary ; dengue ; genetics ; intra-host ; variant},
  booktitle = {},
  journal = {{C}linical {M}icrobiology and {I}nfection},
  volume = {20},
  numero = {3},
  pages = {{O}167--{O}175},
  ISSN = {1198-743{X}},
  year = {2014},
  DOI = {10.1111/1469-0691.12368},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00011617},
}