@article{PAR00011391, title = {{B}artonella melophagi in {M}elophagus ovinus (sheep ked) collected from sheep in northern {O}romia, {E}thiopia}, author = {{K}umsa, {B}. and {P}arola, {P}. and {R}aoult, {D}idier and {S}ocolovschi, {C}.}, editor = {}, language = {{ENG}}, abstract = {{M}elophagus ovinus (sheep ked) is one of the most common ectoparasites that contributes to enormous economic losses in the productivity of sheep in many countries. {T}he present study was conducted from {J}anuary 2012 to {J}uly 2013 on {M}. ovinus collected from sheep at three sites in {E}thiopia. {O}f the sheep studied, 65.7% (88/134) were infested with {M}. ovinus. {T}he prevalence of {M}. ovinus was 76% (76/100), 47% (8/17) and 23.5% (4/17) at the {K}imbibit, {C}hacha and {S}hano sites, respectively. {A}n overall number of 229 {M}. ovinus specimens (138 females, 86 males and five pupae) and 554 {M}. ovinus specimens (272 females, 282 males) were collected from young and adult sheep, respectively. {B}artonella {DNA} was detected in 89% (694/783) of {M}. ovinus using a quantitative {B}artonella genus-specific {PCR} assay targeting the 16{S}/23{S} r{RNA} intergenic spacer region. {T}he sequencing of the {PCR} products of fragments of the glt{A} and rpo{B} genes showed 99.6-100% and 100% homology, respectively, with {B}. melophagi. {S}tatistically significant variation was not noted in the overall prevalence of {B}artonella {DNA} between female and male {M}. ovinus. {A}ll of the sheep infested with {M}. ovinus 100% (88/88) harbored at least one {M}. ovinus specimen that contained {B}artonella {DNA}. {T}his study highlights that {B}. melophagi in {M}. ovinus from sheep in highlands in {E}thiopia possibly has certain zoonotic importance.}, keywords = {{S}heep ; {B}artonella melophagi ; {M}elophagus ovinus ; {S}heep ked ; {M}olecular ; detection ; {O}romia, {E}thiopia ; {ETHYOPIE}}, booktitle = {}, journal = {{C}omparative {I}mmunology {M}icrobiology and {I}nfectious {D}iseases}, volume = {37}, numero = {1}, pages = {69--76}, ISSN = {0147-9571}, year = {2014}, DOI = {10.1016/j.cimid.2013.11.001}, URL = {https://www.documentation.ird.fr/hor/{PAR}00011391}, }