@article{PAR00011283,
  title = {{H}igh-throughput isolation of giant viruses of the {M}imiviridae and {M}arseilleviridae families in the {T}unisian environment},
  author = {{B}oughalmi, {M}. and {S}aadi, {H}. and {P}agnier, {I}. and {C}olson, {P}. and {F}ournous, {G}. and {R}aoult, {D}idier and {L}a {S}cola, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{G}iant viruses of the {M}egavirales order have been recently isolated from aquatic environments and have long been neglected because they are removed from samples during viral purification for viral metagenomic studies. {D}ue to bacterial overgrowth and susceptibility to high concentrations of antibiotics, isolation by amoeba co-culture has a low efficiency and is highly time-consuming. {T}hus, few environments have been exhaustively investigated to date, although the ubiquitous distribution of the {A}canthamoeba sp. suggests that these viruses could also be ubiquitous. {I}n this work, we have implemented a high-throughput method to detect amoebae lysis on agar plates that allows the testing of hundreds of samples in a few days. {U}sing this procedure, a total of 11 new {M}arseilleviridae strains and four new {M}imiviridae strains, including a virus infected with a virophage, were isolated from 1000 environmental samples from {T}unisia. {O}f these, four corresponded to new genotypic variants. {T}hese isolates are the first {A}frican environmental isolates identified from these two families, and several samples were obtained from a hypersaline aquatic environment. {T}hese results demonstrate that this technique can be used for the evaluation and characterization of large collections of giant viruses to provide insight into understanding their ecology.},
  keywords = {{TUNISIE}},
  booktitle = {},
  journal = {{E}nvironmental {M}icrobiology},
  volume = {15},
  numero = {7},
  pages = {2000--2007},
  ISSN = {1462-2912},
  year = {2013},
  DOI = {10.1111/1462-2920.12068},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00011283},
}