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Boughalmi M., Saadi H., Pagnier I., Colson P., Fournous G., Raoult Didier, La Scola B. (2013). High-throughput isolation of giant viruses of the Mimiviridae and Marseilleviridae families in the Tunisian environment. Environmental Microbiology, 15 (7), 2000-2007. ISSN 1462-2912

Lien direct chez l'éditeur doi:10.1111/1462-2920.12068

Titre
High-throughput isolation of giant viruses of the Mimiviridae and Marseilleviridae families in the Tunisian environment
Année de publication2013
Type de documentArticle référencé dans le Web of Science WOS:000328955900007
AuteursBoughalmi M., Saadi H., Pagnier I., Colson P., Fournous G., Raoult Didier, La Scola B.
SourceEnvironmental Microbiology, 2013, 15 (7), p. 2000-2007. ISSN 1462-2912
RésuméGiant viruses of the Megavirales order have been recently isolated from aquatic environments and have long been neglected because they are removed from samples during viral purification for viral metagenomic studies. Due to bacterial overgrowth and susceptibility to high concentrations of antibiotics, isolation by amoeba co-culture has a low efficiency and is highly time-consuming. Thus, few environments have been exhaustively investigated to date, although the ubiquitous distribution of the Acanthamoeba sp. suggests that these viruses could also be ubiquitous. In this work, we have implemented a high-throughput method to detect amoebae lysis on agar plates that allows the testing of hundreds of samples in a few days. Using this procedure, a total of 11 new Marseilleviridae strains and four new Mimiviridae strains, including a virus infected with a virophage, were isolated from 1000 environmental samples from Tunisia. Of these, four corresponded to new genotypic variants. These isolates are the first African environmental isolates identified from these two families, and several samples were obtained from a hypersaline aquatic environment. These results demonstrate that this technique can be used for the evaluation and characterization of large collections of giant viruses to provide insight into understanding their ecology.
Plan de classementEntomologie médicale / Parasitologie / Virologie [052]
Descr. géo.TUNISIE
LocalisationFonds IRD
Identifiant IRDPAR00011283
Lien permanenthttp://www.documentation.ird.fr/hor/PAR00011283

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