@article{PAR00011193,
  title = {{F}irst isolation of a {M}arseillevirus in the diptera {S}yrphidae {E}ristalis tenax},
  author = {{B}oughalmi, {M}. and {P}agnier, {I}. and {A}herfi, {S}. and {C}olson, {P}. and {R}aoult, {D}idier and {L}a {S}cola, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{O}bjective: {G}iant viruses and amoebae are common in freshwater, where they can coexist with various insects. {W}e screened insect larvae to detect giant viruses using a high-throughput method. {M}ethods: {W}e analyzed 86 {E}ristalis tenax larvae obtained from stagnant water reservoirs in {T}unisia. {T}he larvae were decontaminated and then dissected to remove internal parts for coculture with {A}canthamoeba polyphaga. {G}enome sequencing of isolated viruses was performed on a 454 {R}oche instrument, and comparative genomics were performed. {R}esults: {O}ne {M}arseillevirus, named {I}nsectomime virus, was isolated. {T}he genome assembly generated two scaffolds, which were 382,776 and 3,855 bp in length. {A}mong the 477 identified predicted proteins, the best hit for 435 of the identified proteins was a {M}arseillevirus or {L}ausannevirus protein. {T}unisvirus was the most closely related to {I}nsectomime, with 446 orthologs. {O}ne {I}nsectomime protein shared with {L}ausannevirus and {T}unisvirus showed the highest similarity with a protein from an aphid. {C}onclusion: {T}he isolation of a {M}arseillevirus from an insect expands the diversity of environments in which giant viruses have been isolated. {T}he coexistence of larvae and giant viruses in stagnant water may explain the presence of the giant virus in the larva internal structures. {T}his study illustrates the putative role of amoeba in lateral gene transfer not only between the organisms it phagocytoses, but also between organisms living in the same environment.},
  keywords = {{G}iant virus ; {M}arseillevirus ; {M}arseilleviridae ; {E}ristalis tenax},
  booktitle = {},
  journal = {{I}ntervirology},
  volume = {56},
  numero = {6},
  pages = {386--394},
  ISSN = {0300-5526},
  year = {2013},
  DOI = {10.1159/000354560},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00011193},
}