@article{PAR00011178,
  title = {{A} decade of improvements in mimiviridae and marseilleviridae isolation from amoeba},
  author = {{P}agnier, {I}. and {R}eteno, {D}. {G}. {I}. and {S}aadi, {H}. and {B}oughalmi, {M}. and {G}aia, {M}. and {S}limani, {M}. and {N}gounga, {T}. and {B}ekliz, {M}. and {C}olson, {P}. and {R}aoult, {D}idier and {L}a {S}cola, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}ince the isolation of the first giant virus, the {M}imivirus, by {T}.{J}. {R}owbotham in a cooling tower in {B}radford, {UK}, and after its characterisation by our group in 2003, we have continued to develop novel strategies to isolate additional strains. {B}y first focusing on cooling towers using our original time-consuming procedure, we were able to isolate a new lineage of giant virus called {M}arseillevirus and a new {M}imivirus strain called {M}amavirus. {I}n the following years, we have accumulated the world's largest unique collection of giant viruses by improving the use of antibiotic combinations to avoid bacterial contamination of amoeba, developing strategies of preliminary screening of samples by molecular methods, and using a high-throughput isolation method developed by our group. {B}ased on the inoculation of nearly 7,000 samples, our collection currently contains 43 strains of {M}imiviridae (14 in lineage {A}, 6 in lineage {B}, and 23 in lineage {C}) and 17 strains of {M}arseilleviridae isolated from various environments, including 3 of human origin. {T}his study details the procedures used to build this collection and paves the way for the high-throughput isolation of new isolates to improve the record of giant virus distribution in the environment and the determination of their pangenome.},
  keywords = {{M}imivirus ; {M}arseillevirus ; {M}egavirus ; {M}egavirales ; {V}irophage ; {A}canthamoeba},
  booktitle = {},
  journal = {{I}ntervirology},
  volume = {56},
  numero = {6},
  pages = {354--363},
  ISSN = {0300-5526},
  year = {2013},
  DOI = {10.1159/000354556},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00011178},
}