%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Saadi, H.
%A Reteno, D. G. I.
%A Colson, P.
%A Aherfi, S.
%A Minodier, P.
%A Pagnier, I.
%A Raoult, Didier
%A La Scola, B.
%T Shan virus : a new mimivirus isolated from the stool of a Tunisian patient with pneumonia
%D 2013
%L PAR00011173
%G ENG
%J Intervirology
%@ 0300-5526
%K Human stool ; Shan virus ; Pneumonia
%K TUNISIE
%M ISI:000325983600009
%N 6
%P 424-429
%R 10.1159/000354564
%U https://www.documentation.ird.fr/hor/PAR00011173
%V 56
%W Horizon (IRD)
%X Objective: Following the isolation of a Marseillevirus from the stool of a healthy young Senegalese and a Mimivirus from a Tunisian patient with pneumonia, we attempted to isolate other giant viruses of amoebae from a large human stool collection. Methods: During the period 2010-2011, a total of 1,605 stool samples, including 115 from Tunisian patients with pneumonia, were cultured on amoebae. We used a recently developed high-throughput isolation system to detect amoebae plaque lysis on agar plates; this method allows for the testing of 100 samples per plate per week. The giant virus was identified by sequencing of genes conserved in Megavirales. Results: A single giant virus, called Shan, was isolated from the stool of a Tunisian patient with pneumonia who responded poorly to antibiotics. This virus has an icosahedral shape typical of members of the family Mimiviridae and a size of 640 +/- 10 nm. Phylogenetic analyses showed that Shan virus was classified as a member of Mimivirus lineage C that infects amoebae. Conclusion: Only one isolate was obtained in this study, suggesting that giant viruses of amoebae are rare in human stool. The isolation of Shan virus from a patient with pneumonia brings into question the etiological role of this virus and its subsequent release in stool.
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