@article{PAR00011173,
  title = {{S}han virus : a new mimivirus isolated from the stool of a {T}unisian patient with pneumonia},
  author = {{S}aadi, {H}. and {R}eteno, {D}. {G}. {I}. and {C}olson, {P}. and {A}herfi, {S}. and {M}inodier, {P}. and {P}agnier, {I}. and {R}aoult, {D}idier and {L}a {S}cola, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{O}bjective: {F}ollowing the isolation of a {M}arseillevirus from the stool of a healthy young {S}enegalese and a {M}imivirus from a {T}unisian patient with pneumonia, we attempted to isolate other giant viruses of amoebae from a large human stool collection. {M}ethods: {D}uring the period 2010-2011, a total of 1,605 stool samples, including 115 from {T}unisian patients with pneumonia, were cultured on amoebae. {W}e used a recently developed high-throughput isolation system to detect amoebae plaque lysis on agar plates; this method allows for the testing of 100 samples per plate per week. {T}he giant virus was identified by sequencing of genes conserved in {M}egavirales. {R}esults: {A} single giant virus, called {S}han, was isolated from the stool of a {T}unisian patient with pneumonia who responded poorly to antibiotics. {T}his virus has an icosahedral shape typical of members of the family {M}imiviridae and a size of 640 +/- 10 nm. {P}hylogenetic analyses showed that {S}han virus was classified as a member of {M}imivirus lineage {C} that infects amoebae. {C}onclusion: {O}nly one isolate was obtained in this study, suggesting that giant viruses of amoebae are rare in human stool. {T}he isolation of {S}han virus from a patient with pneumonia brings into question the etiological role of this virus and its subsequent release in stool.},
  keywords = {{H}uman stool ; {S}han virus ; {P}neumonia ; {TUNISIE}},
  booktitle = {},
  journal = {{I}ntervirology},
  volume = {56},
  numero = {6},
  pages = {424--429},
  ISSN = {0300-5526},
  year = {2013},
  DOI = {10.1159/000354564},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00011173},
}