@article{PAR00011171,
  title = {{A}lcohol disinfection procedure for isolating giant viruses from contaminated samples},
  author = {{S}limani, {M}. and {P}agnier, {I}. and {B}oughalmi, {M}. and {R}aoult, {D}idier and {L}a {S}cola, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{O}bjective: {G}iant viruses of the {M}egavirales order have been neglected in the literature because they are removed from samples during viral purification for viral metagenomic studies. {I}solation via amoeba coculture has low efficiency and is extremely time-consuming. {T}hus, our objective was to improve {M}egavirales detection and recovery by using a new protocol that will eliminate most bacteria present in environmental samples while preserving giant virus viability. {M}ethods: {I}n this study, we tested the ability of a number of disinfection protocols to kill contaminating bacteria. {T}hese treatments were ethanol, {UV} irradiation, desiccation, glutaraldehyde and thermal shock. {R}esults: {O}f all the treatments, a brief ethanol treatment did not significantly reduce the titer of viable viral particles of {A}canthamoeba polyphaga mimivirus or {M}arseillevirus, whereas it efficiently killed {E}scherichia coli. {T}his treatment was applied to environmental samples that previously tested positive for giant viruses and was shown to eliminate contaminating bacteria, whereas it allowed for the isolation of the giant viruses. {C}onclusion: {O}ur results demonstrate that ethanol treatment can be used to evaluate large collections of environmental samples for the presence of giant viruses and to provide insight into understanding their ecology. {T}his study should also facilitate the isolation of giant viruses using other species of protozoa in addition to {A}canthamoeba spp.},
  keywords = {{G}iant viruses ; {M}egavirales detection ; {D}isinfection protocols},
  booktitle = {},
  journal = {{I}ntervirology},
  volume = {56},
  numero = {6},
  pages = {434--440},
  ISSN = {0300-5526},
  year = {2013},
  DOI = {10.1159/000354566},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00011171},
}