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Slimani M., Pagnier I., Boughalmi M., Raoult Didier, La Scola B. (2013). Alcohol disinfection procedure for isolating giant viruses from contaminated samples. Intervirology, 56 (6), 434-440. ISSN 0300-5526

Lien direct chez l'éditeur doi:10.1159/000354566

Alcohol disinfection procedure for isolating giant viruses from contaminated samples
Année de publication2013
Type de documentArticle référencé dans le Web of Science WOS:000325983600011
AuteursSlimani M., Pagnier I., Boughalmi M., Raoult Didier, La Scola B.
SourceIntervirology, 2013, 56 (6), p. 434-440. ISSN 0300-5526
RésuméObjective: Giant viruses of the Megavirales order have been neglected in the literature because they are removed from samples during viral purification for viral metagenomic studies. Isolation via amoeba coculture has low efficiency and is extremely time-consuming. Thus, our objective was to improve Megavirales detection and recovery by using a new protocol that will eliminate most bacteria present in environmental samples while preserving giant virus viability. Methods: In this study, we tested the ability of a number of disinfection protocols to kill contaminating bacteria. These treatments were ethanol, UV irradiation, desiccation, glutaraldehyde and thermal shock. Results: Of all the treatments, a brief ethanol treatment did not significantly reduce the titer of viable viral particles of Acanthamoeba polyphaga mimivirus or Marseillevirus, whereas it efficiently killed Escherichia coli. This treatment was applied to environmental samples that previously tested positive for giant viruses and was shown to eliminate contaminating bacteria, whereas it allowed for the isolation of the giant viruses. Conclusion: Our results demonstrate that ethanol treatment can be used to evaluate large collections of environmental samples for the presence of giant viruses and to provide insight into understanding their ecology. This study should also facilitate the isolation of giant viruses using other species of protozoa in addition to Acanthamoeba spp.
Plan de classementEntomologie médicale / Parasitologie / Virologie [052] ; Sciences fondamentales / Techniques d'analyse et de recherche [020]
LocalisationFonds IRD
Identifiant IRDPAR00011171
Lien permanenthttp://www.documentation.ird.fr/hor/PAR00011171

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