@article{PAR00011066,
  title = {{V}g m{RNA} induction in an endangered fish species ({A}nguilla anguilla) from the {L}oire estuary ({F}rance)},
  author = {{B}lanchet-{L}etrouve, {I}. and {L}afont, {A}. {G}. and {P}oirier, {L}. and {B}aloche, {S}. and {Z}alouk-{V}ergnoux, {A}. and {D}ufour, {S}ylvie and {M}ouneyrac, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{E}stuarine zones are extremely fragile due to increasing stress from anthropogenic activities. {A}mong those, the {L}oire estuary ({F}rance) is potentially exposed to various contaminants including {E}ndocrine {D}isruptors {C}ompounds ({EDC}s) able to impact the reproduction physiology of fish. {T}he {E}uropean eel ({A}nguilla anguilla), endangered fish species, is apparently not relevant, in its yellow stage, to monitor the effects of endocrine disruption. {D}espite this weakly responsiveness, this study aimed to investigate whether {E}uropean eel from the {L}oire estuary may still be the subject of estrogenic disruption quantifying the hepatic {V}g gene expression according to gender and sexual stage. {V}itellogenin ({V}g) appears as a valuable biomarker of {EDC}s, as well as for exposure and effects. {Q}uantitative real-time {R}everse {T}ranscription {P}olymerase {C}hain {R}eaction (q {RT} {PCR}) was used in this study to amplify responses of hepatic {V}g transcripts. {E}uropean eels were sampled in {M}ay 2009 ({N}=57) and {N}ovember 2010 (during the downstream migration, {N}=10) in two sites of the {L}oire estuary with different ecological conditions and contamination pressures (upstream: {V}arades; downstream: {N}antes). {R}eproductive (gender, sexual maturity stage) and biometric parameters of collected eels were determined. {A} laboratory exposure of silver male to steroid hormones ({T}estosterone ({T}), 11-{K}eto{T}estosterone (11-{KT}), {E}stradiol ({E}2)) was conducted in parallel to validate the q {RT} {PCR} approach on hepatic {V}g m{RNA}. {R}esults demonstrated the responsiveness of exposed silver male eels, since hepatic m{RNA} {V}g induction was observed in {E}2 treated males compared to control specimens. {I}n the field, results of female silver eels reflected large inter-individual differences in the activation of hepatic {V}g at silvering. {H}owever, while only female silver eels should express hepatic {V}g m{RNA}, quantifiable levels were also detected in a proportion of 38% of the other individuals sampled, normally not inclined to express it, those being undifferentiated eels, yellow females, yellow and silver males. {A}ccording to each sexual stage, no difference of expression was observed between eels from the two sampling sites. {H}istological results as well as low {V}g m{RNA} levels detected do not permit a conclusion as to a potential effect of endocrine disruption.},
  keywords = {{E}uropean eel ; {V}itellogenin ; {B}iomarkers ; q {RT} {PCR} ; {FRANCE}},
  booktitle = {},
  journal = {{E}cotoxicology and {E}nvironmental {S}afety},
  volume = {97},
  numero = {},
  pages = {103--113},
  ISSN = {0147-6513},
  year = {2013},
  DOI = {10.1016/j.ecoenv.2013.07.019},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00011066},
}