@article{PAR00011059,
  title = {{I}solation of {T}oscana {V}irus from the {C}erebrospinal {F}luid of a {M}an with {M}eningitis in {M}arseille, {F}rance, 2010},
  author = {{N}ougairede, {A}. and {B}ichaud, {L}. and {T}hiberville, {S}. {D}. and {N}inove, {L}. and {Z}andotti, {C}. and de {L}amballerie, {X}avier and {B}rouqui, {P}. and {C}harrel, {R}. {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}oscana virus ({TOSV}; {B}unyaviridae, {P}hlebovirus) is an emerging arthropod-borne virus transmitted by phlebotomine sandflies. {TOSV} is a frequent cause of central nervous system infection during the warm season in several countries bordering the {M}editerranean {S}ea. {H}ere, we report a case of {TOSV} aseptic meningitis diagnosed in 2012 in {M}arseille, {F}rance. {T}he virus strain was recovered in cell culture from the cerebrospinal fluid. {N}ew-generation sequencing based on {I}on {T}orrent technology was used to determine its complete genome sequence. {P}hylogenetic analysis based on the partial {L} segment revealed that this isolate belongs to the lineage {B} together with other {F}rench, {S}panish, and {M}oroccan strains. {A}lthough several cases of {TOSV} meningitis are reported in the literature, few of them are diagnosed by {RT}-{PCR} combined with virus isolation and further sequence characterization. {T}his case report supports that virus isolation should be attempted whenever possible because this remains the gold standard technique for diagnosis of arthropod-borne viral infections.},
  keywords = {{P}hlebovirus ; {T}oscana virus ; {A}rbovirus ; {S}andfly ; {M}eningitis ; {M}editerranean ; {F}rance ; {D}iagnosis ; {I}solation ; {N}ew-generation sequencing ; {E}mergence ; {FRANCE}},
  booktitle = {},
  journal = {{V}ector-{B}orne and {Z}oonotic {D}iseases},
  volume = {13},
  numero = {9},
  pages = {685--688},
  ISSN = {1530-3667},
  year = {2013},
  DOI = {10.1089/vbz.2013.1316},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00011059},
}