@article{PAR00011054,
  title = {{O}pen membranes are the precursors for assembly of large {DNA} viruses},
  author = {{S}uarez, {C}. and {W}elsch, {S}. and {C}hlanda, {P}. and {H}agen, {W}. and {H}oppe, {S}. and {K}olovou, {A}. and {P}agnier, {I}. and {R}aoult, {D}idier and {L}ocker, {J}. {K}.},
  editor = {},
  language = {{ENG}},
  abstract = {{N}ucleo cytoplasmic large {DNA} viruses ({NCLDV}s) are a group of double-stranded {DNA} viruses that replicate their {DNA} partly or entirely in the cytoplasm in association with viral factories ({VF}s). {T}hey share about 50 genes suggesting that they are derived from a common ancestor. {U}sing transmission electron microscopy ({TEM}) and electron tomography ({ET}) we showed that the {NCLDV} vaccinia virus ({VACV}) acquires its membrane from open membrane intermediates, derived from the {ER}. {T}hese open membranes contribute to the formation of a single open membrane of the immature virion, shaped into a sphere by the assembly of the viral scaffold protein on its convex side. {W}e now compare {VACV} with the {NCLDV} {M}imivirus by {TEM} and {ET} and show that the latter also acquires its membrane from open membrane intermediates that accumulate at the periphery of the cytoplasmic {VF}. {I}n analogy to {VACV} this membrane is shaped by the assembly of a layer on the convexside of its membrane, likely representing the {M}imivirus capsid protein. {B}y quantitative {ET} we show for both viruses that the open membrane intermediates of assembly adopt an open-eight' conformation with a characteristic diameter of 90nm for {M}imi- and 50nm for {VACV}. {W}e discuss these results with respect to the common ancestry of {NCLDV}s and propose a hypothesis on the possible origin of this unusual membrane biogenesis.},
  keywords = {},
  booktitle = {},
  journal = {{C}ellular {M}icrobiology},
  volume = {15},
  numero = {11},
  pages = {1883--1895},
  ISSN = {1462-5814},
  year = {2013},
  DOI = {10.1111/cmi.12156},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00011054},
}