@article{PAR00010822,
  title = {{E}valuation of the diagnostic value of fluorescent in situ hybridization in a rat model of bacterial pneumonia},
  author = {{A}tieh, {T}. and {A}udoly, {G}. and {H}raiech, {S}. and {L}epidi, {H}. and {R}och, {A}. and {R}olain, {J}. {M}. and {R}aoult, {D}idier and {P}apazian, {L}. and {B}regeon, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n severe nosocomial pneumonia, the pathogenic responsibility of bacteria isolated from airways is far from certain, and a lung biopsy is sometimes performed. {H}owever, detection and identification of pathogens are frequently unachieved. {H}ere, we developed a protocol for direct visualization of bacteria within the lung tissue using fluorescent in situ hybridization ({FISH}) in a rat model of {A}cinetobacter baumannii pneumonia. {T}he reference positive diagnosis of bacterial pneumonia was the presence of pathological signs of pneumonia associated with the proof of bacteria or bacterial {PCR} products into the parenchyma. {B}y analysis of 122 sets of slices from 26 rats and using the eubacterial probe {EUB}-338, our results show that {FISH} reached a sensitivity and a diagnostic accuracy higher than that of optic microscopy (sensitivity: 96% versus 55.4% and diagnostic accuracy: 96.7% versus 66.4%), whereas both approaches had 100% specificity. {FISH} could be useful especially on negative biopsies from patients with suspected infectious pneumonia.},
  keywords = {{A}cinetobacter {B}aumannii ; {L}ung infection ; {R}at model ; {F}luorescent in situ hybridization ; {S}ensitivity ; {D}iagnostic},
  booktitle = {},
  journal = {{D}iagnostic {M}icrobiology and {I}nfectious {D}isease},
  volume = {76},
  numero = {4},
  pages = {425--431},
  ISSN = {0732-8893},
  year = {2013},
  DOI = {10.1016/j.diagmicrobio.2013.04.028},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00010822},
}