@article{PAR00010727,
  title = {{M}olecular level characterization of methyl sugars in marine high molecular weight dissolved organic matter},
  author = {{P}anagiotopoulos, {C}. and {R}epeta, {D}. {J}. and {M}athieu, {L}. and {R}ontani, {J}. {F}. and {S}emp{\'e}r{\'e}, {R}ichard},
  editor = {},
  language = {{ENG}},
  abstract = {{D}issolved organic matter ({DOM}) is the largest active organic carbon reservoir in the ocean (662 {GT} {C}), a major fraction (> 95%) of which remains chemically uncharacterized. {T}he concentration and isolation of {DOM} from seawater by ultrafiltration facilitates its chemical characterization by spectroscopic techniques. {U}sing ultrafiltration, silver cation preparative chromatography and gas chromatography coupled with mass spectrometry ({GC}-{MS}), we identified 50 novel sugar compounds after hydrolysis of the high molecular weight dissolved organic matter fraction ({HMWDOM}; the fraction of {DOM} isolated after ultrafiltration). {S}ugars were identified by comparison of their mass spectra with those of chemically synthetized standards and with spectra previously described in the literature. {O}ur results showed that mono- and di-methylated hexoses; mono- and di-methylated pentoses; mono- and di-methylated 6-deoxysugars, as well as heptoses, methylated heptoses, 3,6-dideoxysugars and 1,6 anhydrosugars (levoglucosan, mannosan, and galactosan) are components of {HMWDOM}, which may explain the low apparent yields of sugars recovered by molecular level ({HPLC}) analyses of {HMWDOM} after hydrolysis. {F}rom three depths spanning the surface (15 m) to bathypelagic (1800 m) ocean in the {N}orth {P}acific near {H}awaii our results showed that mono- and di-methylated hexoses were most abundant in the surface sample (64% of the total identified methylated sugar compounds), while at 1800 m monomethylated 6-deoxysugars were the dominant sugars (42% of the total identified methylated sugar compounds). {T}he high diversity of mono- and di-methylated hexoses in the surface sample most likely suggests an algal and/or bacterial source, while the high abundance of methylated 6-deoxy hexoses in the deep sample points toward an important bacterial contribution because the latter sugars are mostly found in bacterial lipopolysaccharides as well as highly degraded organic material.},
  keywords = {{H}igh molecular weight dissolved organic matter ({HMWDOM}) ; {A}cyl polysaccharides ({APS}) ; {M}ethylated sugars ; {H}eptoses ; {A}nhydrosugars ; 3,6-{D}ideoxysugars ; {C}hemical synthesis of methylated sugars ; {GC}-{MS} ; {OCEN} {PACIFIQUE} {NORD} ; {HAWAI}},
  booktitle = {},
  journal = {{M}arine {C}hemistry},
  volume = {154},
  numero = {},
  pages = {34--45},
  ISSN = {0304-4203},
  year = {2013},
  DOI = {10.1016/j.marchem.2013.04.003},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00010727},
}