@article{PAR00010723,
  title = {{I}dentification of rare pathogenic bacteria in a clinical microbiology laboratory : impact of matrix-assisted laser desorption ionization-time of flight mass spectrometry},
  author = {{S}eng, {P}. and {A}bat, {C}. and {R}olain, {J}. {M}. and {C}olson, {P}. and {L}agier, {J}. {C}. and {G}ouriet, {F}. and {F}ournier, {P}. {E}. and {D}rancourt, {M}. and {L}a {S}cola, {B}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{D}uring the past 5 years, matrix-assisted laser desorption ionization-time of flight ({MALDI}-{TOF}) mass spectrometry ({MS}) has become a powerful tool for routine identification in many clinical laboratories. {W}e analyzed our 11-year experience in routine identification of clinical isolates (40 months using {MALDI}-{TOF} {MS} and 91 months using conventional phenotypic identification [{CPI}]). {A}mong the 286,842 clonal isolates, 284,899 isolates of 459 species were identified. {T}he remaining 1,951 isolates were misidentified and required confirmation using a second phenotypic identification for 670 isolates and using a molecular technique for 1,273 isolates of 339 species. {MALDI}-{TOF} {MS} annually identified 112 species, i.e., 36 species/10,000 isolates, compared to 44 species, i.e., 19 species/10,000 isolates, for {CPI}. {O}nly 50 isolates required second phenotypic identifications during the {MALDI}-{TOF} {MS} period (i.e., 4.5 reidentifications/10,000 isolates) compared with 620 isolates during the {CPI} period (i.e., 35.2/10,000 isolates). {W}e identified 128 bacterial species rarely reported as human pathogens, including 48 using phenotypic techniques (22 using {CPI} and 37 using {MALDI}-{TOF} {MS}). {A}nother 75 rare species were identified using molecular methods. {MALDI}-{TOF} {MS} reduced the time required for identification by 55-fold and 169-fold and the cost by 5-fold and 96-fold compared with {CPI} and gene sequencing, respectively. {MALDI}-{TOF} {MS} was a powerful tool not only for routine bacterial identification but also for identification of rare bacterial species implicated in human infectious diseases. {T}he ability to rapidly identify bacterial species rarely described as pathogens in specific clinical specimens will help us to study the clinical burden resulting from the emergence of these species as human pathogens, and {MALDI}-{TOF} {MS} may be considered an alternative to molecular methods in clinical laboratories.},
  keywords = {},
  booktitle = {},
  journal = {{J}ournal of {C}linical {M}icrobiology},
  volume = {51},
  numero = {7},
  pages = {2182--2194},
  ISSN = {0095-1137},
  year = {2013},
  DOI = {10.1128/jcm.00492-13},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00010723},
}