%0 Journal Article %9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES %A Addou, A. N. %A Schumann, P. %A Sproer, C. %A Bouanane-Darenfed, A. %A Amarouche-Yala, S. %A Hacene, H. %A Cayoll, J. L. %A Fardeau, Marie-Laure %T Melghirimyces thermohalophilus sp nov., a thermoactinomycete isolated from an Algerian salt lake %D 2013 %L PAR00010719 %G ENG %J International Journal of Systematic and Evolutionary Microbiology %@ 1466-5026 %K ALGERIE %M ISI:000320741200022 %N 5 %P 1717-1722 %R 10.1099/ijs.0.043760-0 %U https://www.documentation.ird.fr/hor/PAR00010719 %V 63 %W Horizon (IRD) %X A novel filamentous bacterium, designated Nari11A(T), was isolated from soil collected from a salt lake named Chott Melghir, located in north-eastern Algeria. The strain is an aerobic, halophilic, thermotolerant, Gram-stain-positive bacterium, growing at NaCl concentrations between 5 and 20% (w/v) and at 43-60 degrees C and pH 5.0-10.0. The major fatty acids were iso-C-15:0, anteiso-C-15:0 and iso-C-17:0. The DNA G+C content was 53.4 mol%. LL-Diaminopimelic acid was the diamino acid of the peptidoglycan. The major menaquinone was MK-7, but MK-6 and MK-8 were also present in trace amounts. The polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and three unidentified phospholipids. Results of molecular and phenotypic analyses led to the description of the strain as a novel member to the genus Melghirimyces, family Thermoactinomycetaceae. Strain Nari11A(T) shows 96.7% 16S rRNA gene sequence similarity to the type strain of Melghirimyces algeriensis. On the basis of phenotypic, physiological and phylogenetic data, strain Nari11A(T) (=DSM 45514(T) =CCUG 60050(T)) represents the type strain of a novel species, for which the name Melghirimyces thermohalophllus sp. nov. is proposed. %$ 084