@article{PAR00010632,
  title = {{A} {DNA} microarray for the versatile diagnosis of infectious diarrhea},
  author = {{D}onatin, {E}. and {B}uffet, {S}. and {L}eroy, {Q}. and {R}aoult, {D}idier and {D}rancourt, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}everal bacteria, viruses, and parasites cause diarrhea as coinfecting pathogens. {W}e designed a {DNA} microarray comprising 60-bp probes spotted 194times for the multiplex detection of 33 enteropathogenic bacteria and seven enteropathogenic viruses, and the archaeon {M}ethanobrevibacter smithii was used as an internal positive control. {N}ine pathogen-free stool specimens were used as negative controls. {O}ne of these control specimens was further spiked with {S}almonella enterica as a positive control. {T}he microarray was then tested with 40 pathological stool specimens, comprising {S}. enterica (n=30), {C}ampylobacter jejuni (n=4), pathogenic {E}scherichia coli (n=2), and adenovirus (n=4). {M}. smithii was detected in 47/49 (95.9%) specimens, no pathogen was detected in negative controls and {S}. enterica was identified in the {S}. enterica-spiked positive control. {T}he overall specificity was 100% and the overall sensitivity was 97.5% because one {S}. enterica sample was missed by the microarray. {T}he multiplexed detection of {C}. jejuni spiked into an adenovirus-positive stool sample gave positive results, with fluorescence values of 14.3 and 9.1, respectively. {T}hese data indicate that using the protocol developed in this article, the {DNA} array allows for the multiplexed detection of some enteropathogens in stool samples.},
  keywords = {{DNA} microarray ; diagnosis ; infectious diarrhea},
  booktitle = {},
  journal = {{A}pmis},
  volume = {121},
  numero = {7},
  pages = {634--642},
  ISSN = {0903-4641},
  year = {2013},
  DOI = {10.1111/apm.12081},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00010632},
}