@article{PAR00010524,
  title = {{A} versatile medium for cultivating methanogenic archaea},
  author = {{K}helaifia, {S}. and {R}aoult, {D}idier and {D}rancourt, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {M}ethanobrevibacter smithii, {M}ethanobrevibacter oralis, {M}ethanosphaera stadtmanae, {M}ethanomassilicoccus luminyensis and {M}ethanobrevibacter arboriphilicus have been cultured from human digestive microbiota. {E}ach one of these fastidious methanogenic archaea requires a specific medium for its growth, hampering their routine isolation and the culture. {M}ethodology/{P}rincipal {F}indings: {A} new culture medium here referred as {SAB} medium was optimized and tested to cultivate methanogens associated with human microbiota, as well as two mesophile methanogens {M}ethanobacterium beijingense and {M}ethanosaeta concilii. {I}t was further tested for the isolation of archaea from 20 human stool specimens including 10 specimens testing positive for {PCR} detection of {M}. smithii. {A}fter inoculating 10(5) colony-forming-unit archaea/m{L} or 1 g stool specimen in parallel in {SAB} medium and reference {DSMZ} medium in the presence of negative controls, growth of archaea was determined by optical microscopy and the measurement of methane production by gas chromatography. {W}hile the negative controls remained sterile, all tested archaea grew significantly more rapidly in {SAB} medium than in reference medium in 1-3 days ({P}<0.05, {S}tudent test). {A}mong {PCR}-positive stool specimens, 10/10 grew in the {SAB} medium, 6/10 in {DSMZ} 119 medium, 5/10 in {DSMZ} 322 medium and 3/10 in {DSMZ} 334 c medium. {F}our out of ten {PCR}-negative stool specimens grew after a 3-week incubation in the {SAB}-medium whereas no growth was detected in any of the reference media. 16{S} r{RNA} gene sequencing yielded 99-100% sequence similarity with reference {M}. smithii except for one specimen that yielded 99-100% sequence similarity with reference {M}ethanobrevibacter millerae. {C}onclusions/{S}ignificance: {SAB} medium allows for the versatile isolation and growth of methanogenic archaea associated with human gut microbiota including the archaea missed by inoculation of reference media. {I}mplementation of the {SAB} medium in veterinary and medical microbiology laboratories will ease the routine culture-based detection of methanogenic archaea in clinical and environmental specimens.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {8},
  numero = {4},
  pages = {e61563},
  ISSN = {1932-6203},
  year = {2013},
  DOI = {10.1371/journal.pone.0061563},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00010524},
}